Rabbit MMP-9 (Matrix Metalloproteinase 9) ELISA Kit | MyBioSource MBS2502803 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rabbit MMP-9 (Matrix Metalloproteinase 9) ELISA Kit

catalog :

MBS2502803

quantity :

24-Strip-Wells

price :

235 USD

product information

catalog number :

MBS2502803

products type :

ELISA Kit

products full name :

Rabbit MMP-9 (Matrix Metalloproteinase 9) ELISA Kit

products short name :

[MMP-9]

other names :

[matrix metalloproteinase-9 preproprotein; Matrix metalloproteinase-9; matrix metalloproteinase-9; macrophage gelatinase; matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); type V collagenase; matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB]

products gene name :

[MMP-9]

other gene names :

[MMP9; MMP9; GELB; CLG4B; MMP-9; MANDP2; CLG4B; MMP-9; GELB]

uniprot entry name :

MMP9_HUMAN

reactivity :

Rabbit

sequence length :

707

specificity :

This kit recognizes natural and recombinant Rabbit MMP-9. No significant cross-reactivity or interference between Rabbit MMP-9 and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 3.125-200ng/mL. Sensitivity: Min: 1.875ng/mL; Max: 200ng/mL

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rabbit MMP-9 concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MMP-9. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MMP-9 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MMP-9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of MMP-9. You can calculate the concentration of MMP-9 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

74272287

ncbi acc num :

NP_004985.2

ncbi gb acc num :

NM_004994.2

uniprot acc num :

P14780

ncbi mol weight :

78,458 Da

ncbi pathways :

AGE/RAGE Pathway (698754); Activation Of Matrix Metalloproteinases Pathway (576264); Angiogenesis Pathway (198772); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Axon Guidance Pathway (105688); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); CXCR4-mediated Signaling Events Pathway (137910); Collagen Degradation Pathway (730309); Collagen Formation Pathway (645288)

ncbi summary :

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008]

uniprot summary :

MMP9: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly- -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. Exists as monomer or homodimer; disulfide-linked. Exists also as heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form. Interacts with ECM1. Activated by 4-aminophenylmercuric acetate and phorbol ester. Up-regulated by ARHGEF4, SPATA13 and APC via the JNK signaling pathway in colorectal tumor cells. Produced by normal alveolar macrophages and granulocytes. Inhibited by histatin-3 1/24 (histatin-5). Inhibited by ECM1. Belongs to the peptidase M10A family. Protein type: Secreted, signal peptide; Protease; Secreted; EC 3.4.24.35; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 20q13.12. Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region. Molecular Function: collagen binding; identical protein binding; protein binding; zinc ion binding; metalloendopeptidase activity; endopeptidase activity. Biological Process: positive regulation of keratinocyte migration; extracellular matrix disassembly; axon guidance; collagen catabolic process; extracellular matrix organization and biogenesis; ossification; macrophage differentiation; positive regulation of apoptosis; ephrin receptor signaling pathway; proteolysis; leukocyte migration; skeletal development; embryo implantation. Disease: Metaphyseal Anadysplasia 2

size1 :

24-Strip-Wells

price1 :

235 USD

size2 :

48-Strip-Wells

price2 :

450

size3 :

96-Strip-Wells

price3 :

535

size4 :

5x96-Strip-Wells

price4 :

2255

size5 :

10x96-Strip-Wells

price5 :

3975