Human FTH (Ferritin, Heavy Polypeptide) ELISA Kit | MyBioSource MBS2502794 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human FTH (Ferritin, Heavy Polypeptide) ELISA Kit

catalog :

MBS2502794

quantity :

48-Strip-Wells

price :

410 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2502794

products type :

ELISA Kit

products full name :

Human FTH (Ferritin, Heavy Polypeptide) ELISA Kit

products short name :

FTH

other names :

ferritin heavy chain; Ferritin heavy chain; ferritin heavy chain; apoferritin; cell proliferation-inducing gene 15 protein; ferritin H subunit; placenta immunoregulatory factor; proliferation-inducing protein 15; ferritin, heavy polypeptide 1; Cell proliferation-inducing gene 15 proteinFerritin heavy chain, N-terminally processed

products gene name :

FTH

other gene names :

FTH1; FTH1; FHC; FTH; HFE5; PLIF; FTHL6; PIG15; FTH; FTHL6; Ferritin H subunit

uniprot entry name :

FRIH_HUMAN

reactivity :

Human

sequence length :

183

specificity :

This assay has high sensitivity and excellent specificity for detection of Human FTH. No significant cross-reactivity or interference between Human FTH and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human FTH and all the analogues, therefore, cross reaction may still exist.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 12.5--800ng/mL. Sensitivity: 7.5ng/mL

products description :

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FTH in human serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FTH. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human FTH and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FTH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human FTH. You can calculate the concentration of Human FTH in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

56682959

ncbi acc num :

NP_002023.2

ncbi gb acc num :

NM_002032.2

uniprot acc num :

P02794

ncbi mol weight :

21,226 Da

ncbi pathways :

Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Clathrin Derived Vesicle Budding Pathway (119545); Golgi Associated Vesicle Biogenesis Pathway (119546); Iron Metabolism In Placenta Pathway (672461); Iron Uptake And Transport Pathway (187191); Membrane Trafficking Pathway (106160); Mineral Absorption Pathway (212237); Mineral Absorption Pathway (212220); Scavenging By Class A Receptors Pathway (833814); Transmembrane Transport Of Small Molecules Pathway (106572)

ncbi summary :

This gene encodes the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

uniprot summary :

Ferritin-H: Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited. Belongs to the ferritin family. Protein type: Oxidoreductase; EC 1.16.3.1; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll. Chromosomal Location of Human Ortholog: 11q13. Cellular Component: ferritin complex; mitochondrion; cytosol; nucleus. Molecular Function: ferroxidase activity; protein binding; ferric iron binding; iron ion binding. Biological Process: negative regulation of cell proliferation; receptor-mediated endocytosis; negative regulation of fibroblast proliferation; cellular iron ion homeostasis; immune response; post-Golgi vesicle-mediated transport; transmembrane transport; iron ion transport; intracellular sequestering of iron ion. Disease: Hemochromatosis, Type 5

size1 :

48-Strip-Wells

price1 :

410 USD

size2 :

96-Strip-Wells

price2 :

490

more info or order :

MyBioSource product webpage