catalog number :
MBS2502777
products type :
ELISA Kit
products full name :
Human DEFbeta2/DEFB2 (Defensin Beta 2) ELISA Kit
products short name :
DEFbeta2/DEFB2
other names :
Defensin-B2; Defensin-B2
products gene name :
DEFbeta2/DEFB2
other gene names :
DefB2; OaDefB2
uniprot entry name :
DEFB2_ORNAN
specificity :
This kit recognizes natural and recombinant Human DEFbeta2/DEFB2. No significant cross-reactivity or interference between Human DEFbeta2/DEFB2 and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 62.5-4000pg/mL. Sensitivity: The minimum detectable dose of Human DEFbeta2/DEFB2 is 37.5pg/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
products description :
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to DEFbeta2/DEFB2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for DEFbeta2/DEFB2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain DEFbeta2/DEFB2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of DEFbeta2/DEFB2. You can calculate the concentration of DEFbeta2/DEFB2 in the samples by comparing the OD of the samples to the standard curve.
ncbi mol weight :
7,435 Da