ELISA/assay

Mouse CRY1 (Cryptochrome 1) ELISA Kit

MBS2502769

24-Strip-Wells

215 USD

ELISA Kit

Mouse CRY1 (Cryptochrome 1) ELISA Kit

[CRY1]

[Cry1, partial; Cry1; Cry1]

[CRY1]

[cry1]

W0GGX3_BACTU

Mouse

74

This kit recognizes Mouse CORT in samples. No significant cross-reactivity or interference between CORT and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum And Plasma Samples. Assay Type: Quantitative Competitive. Detection Range: 3.12-200ng/mL. Sensitivity: 1.93ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 mouse samples with low, mid range and high level CORT were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 mouse samples with low, mid range and high level CORT were tested on 3 different plates, 20 replicates in each plate.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse CORT concentrations in serum and plasma samples. Please contact tech-support for other sample type detection. Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with CORT. During the reaction, CORT in the sample or standard competes with a fixed amount of CORT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to CORT. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CORT in the samples is then determined by comparing the OD of the samples to the standard curve!!Background/Introduction: Corticosterone, also known as 17-deoxycortisol, it is a steroid hormone secreted by the outer layer, or cortex, of the adrenal gland. Classed as a glucocorticoid, corticosterone helps regulate the conversion of amino acids into carbohydrates and glycogen by the liver, and helps stimulate glycogen formation in the tissues [1]. Corticosterone is similar in structure, although somewhat less potent, than the other glucocorticoids cortisol and cortisone. It is produced in response to stimulation by the pituitary substance adrenocorticotropic hormone (ACTH). In some species, but not in humans, corticosterone is the predominant glucocorticoid secreted by the adrenal. It is a precursor in the synthesis of aldosterone, another adrenal cortical steroid. The increase in corticosterone is linked to anxiety relief. This finding depends on the time at which the administration of corticosterone took place as compared to when the fear conditioning took place, corticosterone can either facilitate or interrupt conditioned fear [2].

572927063

AHF57051.1

8,316 Da

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590