ELISA/assay

Porcine tPA (Plasminogen Activator, Tissue) ELISA Kit

MBS2502752

48-Strip-Wells

450 USD

ELISA Kit

Porcine tPA (Plasminogen Activator, Tissue) ELISA Kit

tPA

tPA, partial; TPA protein; tissue-type plasminogen activator; alteplase; plasminogen activator, tissue type; reteplase; t-plasminogen activator; tissue plasminogen activator (t-PA); plasminogen activator, tissue; TPA protein

tPA

PLAT; tPA; TPA; T-PA

Q6LBF5_HUMAN

Porcine

19

This kit recognizes natural and recombinant Porcine tPA. No significant cross-reactivity or interference between Porcine tPA and analogues was observed.

Store at 4 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.781-50ng/mL. Sensitivity: The minimum detectable dose of Porcine tPA is 0.469ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Porcine tPA concentrations in serum, plasma and other biological fluids.

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to tPA. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for tPA and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain tPA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of tPA. You can calculate the concentration of tPA in the samples by comparing the OD of the samples to the standard curve.

2285954

CAA54669.1

Q6LBF5

2,342 Da

Blood Clotting Cascade Pathway (198840); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Dissolution Of Fibrin Clot Pathway (106061); Endochondral Ossification Pathway (198812); Hemostasis Pathway (106028); Orphan Transporters Pathway (1127546); Selenium Pathway (198825); Senescence And Autophagy Pathway (198780)

This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

48-Strip-Wells

450 USD

96-Strip-Wells

535