ELISA/assay

Human PDCD1LG1 (Programmed Cell Death Protein 1 Ligand 1) ELISA Kit

MBS2502733

48-Strip-Wells

410 USD

ELISA Kit

Human PDCD1LG1 (Programmed Cell Death Protein 1 Ligand 1) ELISA Kit

PDCD1LG1

programmed cell death 1 ligand 1 isoform b; Programmed cell death 1 ligand 1; programmed cell death 1 ligand 1; B7 homolog 1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1; CD274 molecule; B7 homolog 1; B7-H1; CD_antigen: CD274

PDCD1LG1

CD274; CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1; B7H1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1

PD1L1_HUMAN

Human

176

This kit recognizes natural and recombinant Human PDCD1LG1. No significant cross-reactivity or interference between Human PDCD1LG1 and analogues was observed.

Store at 4 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: The minimum detectable dose of Human PDCD1LG1 is 0.094ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human PDCD1LG1 concentrations in serum, plasma and other biological fluids.

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PDCD1LG1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PDCD1LG1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PDCD1LG1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PDCD1LG1. You can calculate the concentration of PDCD1LG1 in the samples by comparing the OD of the samples to the standard curve.

390979639

NP_001254635.1

NM_001267706.1

Q9NZQ7

20,454 Da

Adaptive Immune System Pathway (366160); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Costimulation By The CD28 Family Pathway (119552); Immune System Pathway (106386); PD-1 Signaling Pathway (119557)

CD274: Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 9p24. Cellular Component: integral to membrane; plasma membrane; endomembrane system; external side of plasma membrane. Molecular Function: protein binding. Biological Process: regulation of activated T cell proliferation; cell surface receptor linked signal transduction; negative regulation of interleukin-10 production; negative regulation of interferon-gamma production; negative regulation of activated T cell proliferation; T cell costimulation; immune response; positive regulation of T cell proliferation; signal transduction

48-Strip-Wells

410 USD

96-Strip-Wells

490