Rat VCAM-1 (Vascuolar Cell Adhesion Molecule 1) ELISA Kit | MyBioSource MBS2502676 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat VCAM-1 (Vascuolar Cell Adhesion Molecule 1) ELISA Kit

catalog :

MBS2502676

quantity :

48-Strip-Wells

price :

410 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2502676

products type :

ELISA Kit

products full name :

Rat VCAM-1 (Vascuolar Cell Adhesion Molecule 1) ELISA Kit

products short name :

VCAM-1

other names :

vascular cell adhesion protein 1; Vascular cell adhesion protein 1; vascular cell adhesion protein 1; V-CAM 1; VCAM-1; vascular cell adhesion molecule 1; CD_antigen: CD106

products gene name :

VCAM-1/CD106

other gene names :

Vcam1; Vcam1; VCAM1B; Vcam-1; V-CAM 1; VCAM-1

uniprot entry name :

VCAM1_RAT

reactivity :

Rat

sequence length :

739

specificity :

This kit recognizes natural and recombinant Rat VCAM-1/CD106. No significant cross-reactivity or interference between Rat VCAM-1/CD106 and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 12.5-800pg/mL. Sensitivity: The minimum detectable dose of Rat VCAM-1/CD106 is 7.5pg/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).

other info2 :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat VCAM-1/CD106 concentrations in serum, plasma and other biological fluids.

products description :

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to VCAM-1/CD106. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for VCAM-1/CD106 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain VCAM-1/CD106, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of VCAM-1/CD106. You can calculate the concentration of VCAM-1/CD106 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

6981700

ncbi acc num :

NP_037021.1

ncbi gb acc num :

NM_012889.1

uniprot acc num :

P29534

ncbi mol weight :

81,246 Da

ncbi pathways :

Adaptive Immune System Pathway (1121508); African Trypanosomiasis Pathway (194377); African Trypanosomiasis Pathway (194323); Cell Adhesion Molecules (CAMs) Pathway (83461); Cell Adhesion Molecules (CAMs) Pathway (480); Extracellular Matrix Organization Pathway (1121481); HTLV-I Infection Pathway (373894); HTLV-I Infection Pathway (373889); Immune System Pathway (1121507); Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway (1121534)

ncbi summary :

binds integrin very late antigen (VLA4); plays a role in cell adhesion [RGD, Feb 2006]

uniprot summary :

VCAM1: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion. Cellular Component: Golgi apparatus; extracellular space; microvillus; cell surface; endoplasmic reticulum; apical part of cell; early endosome; integral to membrane; podosome; sarcolemma; filopodium; external side of plasma membrane. Molecular Function: integrin binding; amine oxidase activity; cell adhesion molecule binding. Biological Process: response to nicotine; chronic inflammatory response; viral reproduction; heart development; cell-matrix adhesion; response to lipopolysaccharide; heterophilic cell adhesion; leukocyte adhesion; response to ethanol; response to zinc ion; membrane to membrane docking; response to hypoxia; positive regulation of T cell proliferation; response to ionizing radiation; amine metabolic process; cell adhesion; leukocyte tethering or rolling; acute inflammatory response; response to nutrient; aging

size1 :

48-Strip-Wells

price1 :

410 USD

size2 :

96-Strip-Wells

price2 :

490

more info or order :

MyBioSource product webpage