ELISA/assay

Human CDK9 (Cyclin Dependent Kinase 9) ELISA Kit

MBS2502653

24-Strip-Wells

215 USD

ELISA Kit

Human CDK9 (Cyclin Dependent Kinase 9) ELISA Kit

[CDK9]

[cyclin-dependent kinase 9; Cyclin-dependent kinase 9; cyclin-dependent kinase 9; CDC2-related kinase; cell division cycle 2-like protein kinase 4; cell division protein kinase 9; serine/threonine protein kinase PITALRE; tat-associated kinase complex catalytic subunit; cyclin-dependent kinase 9; C-2K; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; Serine/threonine-protein kinase PITALRE; Tat-associated kinase complex catalytic subunit]

[CDK-9]

[CDK9; CDK9; TAK; C-2k; CTK1; CDC2L4; PITALRE; CDC2L4; TAK]

CDK9_HUMAN

Human

372

This kit recognizes natural and some recombinant Human CDK9. No significant crossreactivity or interference between Human CDK9 and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 1.563-100ng/mL. Sensitivity: Min: 0.938ng/mL; Max: 100ng/mL

Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Human CDK9 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Human CDK9. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Human CDK9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Human CDK9, biotinylated detection antibody and Avidin- HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human CDK9. The concentration of Human CDK9 in samples can be calculated by comparing the OD of the samples with the standard curve.

4502747

NP_001252.1

NM_001261.3

P50750

53,365 Da

Disease Pathway (530764); Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway (161052); Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway (106004); Formation Of RNA Pol II Elongation Complex Pathway (105950); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); HIV Infection Pathway (105983); HIV Life Cycle Pathway (105984); HIV Transcription Elongation Pathway (106001); HIV Elongation Arrest And Recovery Pathway (161051)

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. [provided by RefSeq, Jul 2008]

CDK9: a protein kinase of the CDK family. Forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. A component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. Transcriptional elongation factor and cofactor for HIV Tat protein; RNAi blocks HIV replication, and inhibitors also block varicella zoster replication. Mediates signals leading to cardiac hypertrophy. Inhibitor: Flavopiridol. Protein type: Cell cycle regulation; EC 2.7.11.23; Nuclear receptor co-regulator; Protein kinase, CMGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.22; CMGC group; CDK family; CDK/CDK9 subfamily; CDK9 subfamily. Chromosomal Location of Human Ortholog: 9q34.1. Cellular Component: nucleoplasm; transcription elongation factor complex; PML body; transcription elongation factor complex b; intracellular membrane-bound organelle; membrane; cytoplasm. Molecular Function: RNA polymerase subunit kinase activity; protein binding; snRNA binding; DNA binding; cyclin-dependent protein kinase activity; chromatin binding; ATP binding; protein kinase activity. Biological Process: regulation of histone modification; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; transcription, DNA-dependent; positive regulation of histone phosphorylation; positive regulation of viral transcription; regulation of mitotic cell cycle; DNA repair; protein amino acid phosphorylation; regulation of DNA repair; cell proliferation; transforming growth factor beta receptor signaling pathway; RNA elongation from RNA polymerase II promoter; gene expression; positive regulation of transcription from RNA polymerase II promoter; replication fork arrest

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590