Human ARO (Aromatase) ELISA Kit | MyBioSource MBS2502650 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human ARO (Aromatase) ELISA Kit

catalog :

MBS2502650

quantity :

48-Strip-Wells

price :

410 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2502650

products type :

ELISA Kit

products full name :

Human ARO (Aromatase) ELISA Kit

products short name :

ARO

other names :

aromatase; Aromatase; aromatase; cytochrome P-450AROM; cytochrome P450 19A1; cytochrome P450, subfamily XIX (aromatization of androgens); estrogen synthase; estrogen synthetase; flavoprotein-linked monooxygenase; microsomal monooxygenase; cytochrome P450, family 19, subfamily A, polypeptide 1; CYPXIX; Cytochrome P-450AROM; Cytochrome P450 19A1; Estrogen synthase

products gene name :

ARO

other gene names :

CYP19A1; CYP19A1; ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM; ARO1; CYAR; CYP19

uniprot entry name :

CP19A_HUMAN

reactivity :

Human

sequence length :

503

specificity :

This kit recognizes natural and recombinantHumanARO. No significant cross-reactivity or interference between HumanARO and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Quantitative Sandwich. Sensitivity: 0.094ng/mL

products description :

Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanAROconcentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ARO. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for AROand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ARO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ARO.You can calculate the concentration of AROin the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

13904860

ncbi acc num :

NP_112503.1

ncbi gb acc num :

NM_031226.2

uniprot acc num :

P11511

ncbi mol weight :

24,518 Da

ncbi pathways :

Biological Oxidations Pathway (105698); C19/C18-Steroid Hormone Biosynthesis, Pregnenolone = Androstenedione = Estrone Pathway (413383); C19/C18-Steroid Hormone Biosynthesis, Pregnenolone = Androstenedione = Estrone Pathway (468268); Cytochrome P450 - Arranged By Substrate Type Pathway (105700); Defective CYP11A1 Causes Adrenal Insufficiency, Congenital, With 46,XY Sex Reversal (AICSR) Pathway (1127640); Defective CYP11B1 Causes Adrenal Hyperplasia 4 (AH4) Pathway (1127641); Defective CYP11B2 Causes Corticosterone Methyloxidase 1 Deficiency (CMO-1 Deficiency) Pathway (1127642); Defective CYP17A1 Causes Adrenal Hyperplasia 5 (AH5) Pathway (1127643); Defective CYP19A1 Causes Aromatase Excess Syndrome (AEXS) Pathway (1127644); Defective CYP1B1 Causes Glaucoma Pathway (1127645)

ncbi summary :

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

uniprot summary :

CYP19A1: Catalyzes the formation of aromatic C18 estrogens from C19 androgens. Belongs to the cytochrome P450 family. Protein type: Lipid Metabolism - androgen and estrogen; Oxidoreductase; EC 1.14.14.14. Chromosomal Location of Human Ortholog: 15q21.1. Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum. Molecular Function: electron carrier activity; iron ion binding; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; oxygen binding. Biological Process: steroid metabolic process; xenobiotic metabolic process; androgen metabolic process; estrogen biosynthetic process; steroid biosynthetic process; sterol metabolic process. Disease: Aromatase Deficiency; Aromatase Excess Syndrome

size1 :

48-Strip-Wells

price1 :

410 USD

size2 :

96-Strip-Wells

price2 :

490

more info or order :

MyBioSource product webpage