catalog number :
MBS2502648
products type :
ELISA Kit
products full name :
Human LRG1 (Leucine Rich Alpha-2-Glycoprotein 1) ELISA Kit
products short name :
LRG1
other names :
leucine-rich alpha-2-glycoprotein; Leucine-rich alpha-2-glycoprotein; leucine-rich alpha-2-glycoprotein; 1300008B03Rik; 2310031E04Rik; leucine rich alpha 2 glycoprotein; leucine-rich alpha-2-glycoprotein 1
products gene name :
LRG1
other gene names :
LRG1; LRG1; LRG; HMFT1766; LRG; LRG
uniprot entry name :
A2GL_HUMAN
specificity :
This kit recognizes natural and recombinant Human LRG1. No significant cross-reactivity or interference between Human LRG1 and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 7.813-500ng/mL. Sensitivity: 4.688ng/mL
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human LRG1 concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to LRG1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for LRG1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain LRG1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of LRG1. You can calculate the concentration of LRG1 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_443204.1
ncbi gb acc num :
NM_052972.2
ncbi mol weight :
38,178 Da
ncbi summary :
The leucine-rich repeat (LRR) family of proteins, including LRG1, have been shown to be involved in protein-protein interaction, signal transduction, and cell adhesion and development. LRG1 is expressed during granulocyte differentiation (O'Donnell et al., 2002 [PubMed 12223515]).[supplied by OMIM, Mar 2008]
uniprot summary :
LRG1: . Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 19p13.3. Cellular Component: extracellular space; membrane; extracellular region. Molecular Function: transforming growth factor beta receptor binding. Biological Process: positive regulation of angiogenesis; positive regulation of transforming growth factor beta receptor signaling pathway; brown fat cell differentiation; positive regulation of endothelial cell proliferation