Rat PACAP-38(Pituitary Adenylate Cyclase Activating Polypeptide 38)ELISA Kit | MyBioSource MBS2502629 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat PACAP-38(Pituitary Adenylate Cyclase Activating Polypeptide 38)ELISA Kit

catalog :

MBS2502629

quantity :

48-Strip-Wells

price :

410 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2502629

products type :

ELISA Kit

products full name :

Rat PACAP-38(Pituitary Adenylate Cyclase Activating Polypeptide 38)ELISA Kit

products short name :

PACAP-38

other names :

pituitary adenylate cyclase-activating polypeptide type I receptor isoform 4; Pituitary adenylate cyclase-activating polypeptide type I receptor; pituitary adenylate cyclase-activating polypeptide type I receptor; PACAP receptor 1; PACAP type I receptor; PACAP-R1; pituitary adenylate cyclase activating polypeptide 1 receptor type I Hiphop; adenylate cyclase activating polypeptide 1 (pituitary) receptor type I

products gene name :

PACAP-38

other gene names :

ADCYAP1R1; ADCYAP1R1; PAC1; PAC1R; PACAPR; PACAPRI; PACAP type I receptor; PACAP-R-1; PACAP-R1

uniprot entry name :

PACR_HUMAN

reactivity :

Rat

sequence length :

447

specificity :

This assay has high sensitivity and excellent specificity for detection of Rat PACAP-38. No significant cross-reactivity or interference between Rat PACAP-38 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Rat PACAP-38 and all the analogues, therefore, cross reaction may still exist.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Competitive. Detection Range: 7.813--500pg/mL. Sensitivity: 4.688pg/mL

products description :

Description: The kit is a competitive enzyme immunoassay for in vitro quantitative measurement of PACAP-38 in Rat serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Rat PACAP-38. During the reaction, Rat PACAP-38 in the sample or standard competes with a fixed amount of Rat PACAP-38 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat PACAP-38. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Rat PACAP-38 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

313851023

ncbi acc num :

NP_001186566.1

ncbi gb acc num :

NM_001199637.1

uniprot acc num :

P41586

ncbi mol weight :

46,957 Da

ncbi pathways :

Activation Of TRKA Receptors Pathway (106456); Circadian Entrainment Pathway (698773); Circadian Entrainment Pathway (699872); Class B/2 (Secretin Family Receptors) Pathway (106378); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class B Secretin-like Pathway (198781)

ncbi summary :

This gene encodes type I adenylate cyclase activating polypeptide receptor, which is a membrane-associated protein and shares significant homology with members of the glucagon/secretin receptor family. This receptor mediates diverse biological actions of adenylate cyclase activating polypeptide 1 and is positively coupled to adenylate cyclase. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Dec 2010]

uniprot summary :

ADCYAP1R1: This is a receptor for PACAP-27 and PACAP-38. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. May regulate the release of adrenocorticotropin, luteinizing hormone, growth hormone, prolactin, epinephrine, and catecholamine. May play a role in spermatogenesis and sperm motility. Causes smooth muscle relaxation and secretion in the gastrointestinal tract. Belongs to the G-protein coupled receptor 2 family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR; GPCR, family 2. Chromosomal Location of Human Ortholog: 7p14. Cellular Component: neuron projection; tight junction; cell surface; rough endoplasmic reticulum; integral to plasma membrane; plasma membrane; caveola; receptor complex; endosome. Molecular Function: neuropeptide binding; ADP-ribosylation factor binding; vasoactive intestinal polypeptide receptor activity; protein binding; receptor activity; adenylate cyclase binding. Biological Process: response to drug; development of primary female sexual characteristics; nerve growth factor receptor signaling pathway; adenylate cyclase activation; response to estradiol stimulus; G-protein coupled receptor protein signaling pathway; cAMP-mediated signaling; cell surface receptor linked signal transduction; response to ethanol; phospholipase C activation; spermatogenesis; cell differentiation; multicellular organismal response to stress; transmembrane receptor protein tyrosine kinase signaling pathway

size1 :

48-Strip-Wells

price1 :

410 USD

size2 :

96-Strip-Wells

price2 :

490

more info or order :

MyBioSource product webpage