Monkey LCN2 (Lipocalin 2) ELISA Kit | MyBioSource MBS2502545 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Monkey LCN2 (Lipocalin 2) ELISA Kit

catalog :

MBS2502545

quantity :

48-Strip-Wells

price :

450 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2502545

products type :

ELISA Kit

products full name :

Monkey LCN2 (Lipocalin 2) ELISA Kit

products short name :

LCN2

other names :

LCN2, partial; Neutrophil gelatinase-associated lipocalin; neutrophil gelatinase-associated lipocalin; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; lipocalin-2; migration-stimulating factor inhibitor; oncogene 24p3; p25; siderocalin LCN2; lipocalin 2; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Lipocalin-2; Oncogene 24p3; Siderocalin LCN2; p25

products gene name :

LCN2

other gene names :

LCN2; LCN2; 24p3; MSFI; NGAL; HNL; NGAL; NGAL

uniprot entry name :

NGAL_HUMAN

reactivity :

Monkey

sequence length :

198

specificity :

This kit recognizes natural and recombinant Monkey LCN2. No significant cross-reactivity or interference between Monkey LCN2 and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: 0.188ng/mL

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Monkey LCN2 concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to LCN2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for LCN2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain LCN2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of LCN2. You can calculate the concentration of LCN2 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

49457137

ncbi acc num :

CAG46889.1

uniprot acc num :

P80188

ncbi mol weight :

22,457 Da

ncbi pathways :

Iron Uptake And Transport Pathway (187191); Orphan Transporters Pathway (1127546); Transmembrane Transport Of Small Molecules Pathway (106572)

uniprot summary :

LCN2: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. Belongs to the calycin superfamily. Lipocalin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 9q34. Cellular Component: extracellular space; extracellular region; cytosol. Molecular Function: protein homodimerization activity; protease binding; transporter activity; iron ion binding. Biological Process: response to drug; cellular response to nutrient levels; cellular iron ion homeostasis; response to virus; positive regulation of cell projection organization and biogenesis; siderophore transport; innate immune response; ion transport; response to herbicide; transmembrane transport

size1 :

48-Strip-Wells

price1 :

450 USD

size2 :

96-Strip-Wells

price2 :

535

more info or order :

MyBioSource product webpage