ELISA/assay

Human GPC3 (Glypican 3) ELISA Kit

MBS2502526

48-Strip-Wells

410 USD

ELISA Kit

Human GPC3 (Glypican 3) ELISA Kit

GPC3

glypican-3 isoform 4; Glypican-3; glypican-3; glypican proteoglycan 3; heparan sulphate proteoglycan; intestinal protein OCI-5; secreted glypican-3; glypican 3; GTR2-2; Intestinal protein OCI-5; MXR7Secreted glypican-3

GPC3

GPC3; GPC3; SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2; OCI5

GPC3_HUMAN

Human

526

This assay has high sensitivity and excellent specificity for detection of Human GPC3. No significant cross-reactivity or interference between Human GPC3 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human GPC3 and all the analogues, therefore, cross reaction may still exist.

Store at 4 degree C.

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156--10ng/mL. Sensitivity: 0.094ng/mL

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GPC3 in human serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GPC3. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human GPC3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GPC3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human GPC3. You can calculate the concentration of Human GPC3 in the samples by comparing the OD of the samples to the standard curve.

257471010

NP_001158091.1

NM_001164619.1

P51654

68,414 Da

A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway (645305); Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (645308); Defective B3GAT3 Causes JDSSDHD Pathway (1127588); Defective B4GALT1 Causes B4GALT1-CDG (CDG-2d) Pathway (1127592); Defective B4GALT7 Causes EDS, Progeroid Type Pathway (1127587); Defective CHST14 Causes EDS, Musculocontractural Type Pathway (1127591); Defective CHST3 Causes SEDCJD Pathway (1127590); Defective CHST6 Causes MCDC1 Pathway (1127593); Defective CHSY1 Causes TPBS Pathway (1127589); Defective EXT1 Causes Exostoses 1, TRPS2 And CHDS Pathway (1127594)

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]

GPC3: Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1); also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies. Belongs to the glypican family. Protein type: Motility/polarity/chemotaxis; Membrane protein, GPI anchor. Chromosomal Location of Human Ortholog: Xq26.1. Cellular Component: proteinaceous extracellular matrix; extracellular space; lysosomal lumen; anchored to plasma membrane; Golgi lumen; integral to plasma membrane; plasma membrane. Molecular Function: heparan sulfate proteoglycan binding; protein binding. Biological Process: phototransduction, visible light; anatomical structure morphogenesis; glycosaminoglycan metabolic process; negative regulation of peptidase activity; positive regulation of endocytosis; pathogenesis; osteoclast differentiation; embryonic hindlimb morphogenesis; body morphogenesis; bone mineralization; chondroitin sulfate metabolic process; glycosaminoglycan biosynthetic process; positive regulation of glucose import; glycosaminoglycan catabolic process; ureteric bud branching; negative regulation of smoothened signaling pathway; carbohydrate metabolic process; positive regulation of protein catabolic process; positive regulation of smoothened signaling pathway; retinoid metabolic process; positive regulation of BMP signaling pathway; negative regulation of growth; anterior/posterior axis specification; negative regulation of epithelial cell proliferation; lung development. Disease: Simpson-golabi-behmel Syndrome, Type 1; Wilms Tumor 1

48-Strip-Wells

410 USD

96-Strip-Wells

490