catalog number :
MBS2502482
products type :
ELISA Kit
products full name :
Mouse CIC (Circulating Immune Complexes) ELISA Kit
products short name :
CIC
other names :
capicua protein; Protein capicua homolog
other gene names :
CIC; KIAA0306
uniprot entry name :
CIC_HUMAN
specificity :
This kit recognizes natural and recombinant Mouse CIC. No significant cross-reactivity or interference between Mouse CIC and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 15.625-1000ng/mL. Sensitivity: 9.375ng/mL
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse CIC concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CIC. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CIC and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CIC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CIC. You can calculate the concentration of CIC in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
AAK73515.1
ncbi mol weight :
163,820 Da
uniprot summary :
capicua: involved in cerebellar development and ErbB signaling. Significantly expressed in medulloblastomas. Protein type: Transcription factor; DNA-binding. Chromosomal Location of Human Ortholog: 19q13.2. Cellular Component: nucleus. Molecular Function: protein binding; DNA binding; chromatin binding. Biological Process: transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; alveolus development