Mouse TRACP-5b (Tartrate-Resistant Acid Phosphatase 5b) ELISA Kit | MyBioSource MBS2502462 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse TRACP-5b (Tartrate-Resistant Acid Phosphatase 5b) ELISA Kit

catalog :

MBS2502462

quantity :

24-Strip-Wells

price :

215 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2502462

products type :

ELISA Kit

products full name :

Mouse TRACP-5b (Tartrate-Resistant Acid Phosphatase 5b) ELISA Kit

products short name :

[TRACP-5b]

other names :

[tartrate-resistant acid phosphatase type 5; Tartrate-resistant acid phosphatase type 5; tartrate-resistant acid phosphatase type 5; TrATPase; tartrate-resistant acid ATPase; acid phosphatase 5, tartrate resistant; Tartrate-resistant acid ATPase; TrATPase; Type 5 acid phosphatase]

products gene name :

[TRACP-5b]

other gene names :

[ACP5; ACP5; TRAP; SPENCDI; TR-AP; TrATPase]

uniprot entry name :

PPA5_HUMAN

reactivity :

Mouse

sequence length :

325

specificity :

This kit recognizes natural and some recombinant Mouse TRACP-5b. No significant crossreactivity or interference between Mouse TRACP-5b and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.781-50ng/mL. Sensitivity: Min: 0.469ng/mL; Max: 50ng/mL

products description :

Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Mouse TRACP-5b concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Mouse TRACP-5b. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Mouse TRACP-5b and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Mouse TRACP-5b, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse TRACP-5b. The concentration of Mouse TRACP-5b in samples can be calculated by comparing the OD of the samples with the standard curve.

ncbi gi num :

161377455

ncbi acc num :

NP_001104506.1

ncbi gb acc num :

NM_001111036.1

uniprot acc num :

P13686

ncbi mol weight :

36,599 Da

ncbi pathways :

Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway (906000); Defective BTD Causes Biotidinase Deficiency Pathway (906015); Defective CD320 Causes Methylmalonic Aciduria Pathway (906012); Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway (906001); Defective GIF Causes Intrinsic Factor Deficiency Pathway (906004); Defective HLCS Causes Multiple Carboxylase Deficiency Pathway (906014); Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway (906003); Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway (906010); Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway (906009); Defective MMACHC Causes Methylmalonic Aciduria And Homocystinuria Type CblC Pathway (906005)

ncbi summary :

This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. [provided by RefSeq, Aug 2008]

uniprot summary :

ACP5: Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias. Defects in ACP5 are the cause of spondyloenchondrodysplasia with immune dysregulation (SPENCDI). A disease characterized by vertebral and metaphyseal dysplasia, spasticity with cerebral calcifications, and strong predisposition to autoimmune diseases. The skeletal dysplasia is characterized by radiolucent and irregular spondylar and metaphyseal lesions that represent islands of chondroid tissue within bone. ACP5 inactivating mutations result in a functional excess of phosphorylated osteopontin causing deregulation of osteopontin signaling and consequential autoimmune disease. Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family. Protein type: Phosphatase; EC 3.1.3.2; Cofactor and Vitamin Metabolism - riboflavin; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 19p13.2. Cellular Component: lysosome; integral to membrane; cytosol. Molecular Function: acid phosphatase activity; ferric iron binding; ferrous iron binding. Biological Process: negative regulation of interleukin-12 production; vitamin metabolic process; negative regulation of nitric oxide biosynthetic process; response to lipopolysaccharide; negative regulation of superoxide release; negative regulation of interleukin-1 beta production; negative regulation of tumor necrosis factor production; riboflavin metabolic process; defense response to Gram-positive bacterium; dephosphorylation; response to cytokine stimulus; negative regulation of inflammatory response; water-soluble vitamin metabolic process; bone resorption. Disease: Spondyloenchondrodysplasia With Immune Dysregulation

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590

more info or order :

MyBioSource product webpage