catalog number :
MBS2502432
products type :
ELISA Kit
products full name :
Human FcalphaR/CD89 (Receptor I for the Fc Fragment of IgA) ELISA Kit
products short name :
[FcalphaR/CD89]
other names :
[Fc alpha receptor; Immunoglobulin alpha Fc receptor; immunoglobulin alpha Fc receptor; Fc alpha receptor; Fc fragment of IgA, receptor for; CD_antigen: CD89]
products gene name :
[FcalphaR/CD89]
other gene names :
[FCAR; FCAR; CD89; FcalphaRI; CD89; IgA Fc receptor]
uniprot entry name :
FCAR_HUMAN
specificity :
This kit recognizes natural and recombinant Human FcalphaR/CD89. No significant cross-reactivity or interference between Human FcalphaR/CD89 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.781-50ng/mL. Sensitivity: Min: 0.469ng/mL; Max: 50ng/mL
other info2 :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human FcalphaR/CD89 concentrations in serum, plasma and other biological fluids.
products description :
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to FcalphaR/CD89. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for FcalphaR/CD89 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain FcalphaR/CD89, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of FcalphaR/CD89. You can calculate the concentration of FcalphaR/CD89 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
AAC50595.1
ncbi mol weight :
28,343 Da
ncbi pathways :
Phagosome Pathway (153910); Phagosome Pathway (153859); Staphylococcus Aureus Infection Pathway (172846); Staphylococcus Aureus Infection Pathway (171867)
ncbi summary :
This gene is a member of the immunoglobulin gene superfamily and encodes a receptor for the Fc region of IgA. The receptor is a transmembrane glycoprotein present on the surface of myeloid lineage cells such as neutrophils, monocytes, macrophages, and eosinophils, where it mediates immunologic responses to pathogens. It interacts with IgA-opsonized targets and triggers several immunologic defense processes, including phagocytosis, antibody-dependent cell-mediated cytotoxicity, and stimulation of the release of inflammatory mediators. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
uniprot summary :
FCAR: Binds to the Fc region of immunoglobulins alpha. Mediates several functions including cytokine production. 11 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Immunoglobulin superfamily. Chromosomal Location of Human Ortholog: 19q13.42. Cellular Component: integral to plasma membrane; extracellular region; plasma membrane. Molecular Function: IgA binding. Biological Process: immune response
size5 :
10x96-Strip-Wells