catalog number :
MBS2502409
products type :
ELISA Kit
products full name :
DHT (Dihydrotestosterone) ELISA Kit
products short name :
DHT
other names :
Dht; Dht; Dht
uniprot entry name :
G8QBC4_PSEFL
specificity :
This kit recognizes natural and recombinant DHT. No significant cross-reactivity or interference between DHT and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Competitive. Detection Range: 39.063-2500pg/mL. Sensitivity: 23.438pg/mL
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of DHT concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with DHT. During the reaction, DHT in the sample or standard competes with a fixed amount of DHT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to DHT. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of DHT in the samples is then determined by comparing the OD of the samples to the standard curve.
ncbi acc num :
AEV62371.1
ncbi mol weight :
52,403 Da