catalog number :
MBS2502399
products type :
ELISA Kit
products full name :
Rat HMG-CoAR(3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase)ELISA Kit
products short name :
HMG-CoAR
other names :
HMG CoA-reductase, partial; 3-hydroxy-3-methylglutaryl coenzyme A reductase; HMG CoA-reductase
products gene name :
HMG-CoAR
other gene names :
hmg-coa; HMG-CoA;
uniprot entry name :
A5HL74_ORYLA
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat HMG-CoAR. No significant cross-reactivity or interference between Rat HMG-CoAR and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Rat HMG-CoAR and all the analogues, therefore, cross reaction may still exist.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.313--20ng/mL. Sensitivity: 0.188ng/mL
products description :
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of HMG-CoAR in Rat serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat HMG-CoAR. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat HMG-CoAR and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HMG-CoAR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat HMG-CoAR. You can calculate the concentration of Rat HMG-CoAR in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
ABQ09275.1
ncbi mol weight :
42,979 Da
ncbi pathways :
C5 Isoprenoid Biosynthesis, Mevalonate Pathway (750618); C5 Isoprenoid Biosynthesis, Mevalonate Pathway (468288); Metabolic Pathways (750373); Terpenoid Backbone Biosynthesis Pathway (750305); Terpenoid Backbone Biosynthesis Pathway (408)