Human DKK1 (Dickkopf Related Protein 1) ELISA Kit | MyBioSource MBS2502389 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human DKK1 (Dickkopf Related Protein 1) ELISA Kit

catalog :

MBS2502389

quantity :

48-Strip-Wells

price :

410 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2502389

products type :

ELISA Kit

products full name :

Human DKK1 (Dickkopf Related Protein 1) ELISA Kit

products short name :

DKK1

other names :

dickkopf-related protein 1; Dickkopf-related protein 1; dickkopf-related protein 1; dickkopf 1 homolog; dickkopf related protein-1; dickkopf-1 like; dickkopf-like protein 1; hDkk-1; dickkopf WNT signaling pathway inhibitor 1; SK

products gene name :

DKK1

other gene names :

DKK1; DKK1; SK; DKK-1; Dickkopf-1; Dkk-1; hDkk-1

uniprot entry name :

DKK1_HUMAN

reactivity :

Human

sequence length :

266

specificity :

This kit recognizes natural and recombinant Human DKK1. No significant cross-reactivity or interference between Human DKK1 and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: The minimum detectable dose of Human DKK1 is 18.75pg/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).

other info2 :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human DKK1 concentrations in serum, plasma and other biological fluids.

products description :

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to DKK1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for DKK1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain DKK1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of DKK1. You can calculate the concentration of DKK1 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

7110719

ncbi acc num :

NP_036374.1

ncbi gb acc num :

NM_012242.2

uniprot acc num :

O94907

ncbi mol weight :

28,672 Da

ncbi pathways :

Cardiac Progenitor Differentiation Pathway (712094); Direct P53 Effectors Pathway (137939); Disease Pathway (530764); Presenilin Action In Notch And Wnt Signaling Pathway (137914); RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway (1127632); Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway (169352); Signal Transduction Pathway (477114); Signaling By WNT In Cancer Pathway (1127614); Signaling By Wnt Pathway (106510); TCF Dependent Signaling In Response To WNT Pathway (1127516)

ncbi summary :

This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. [provided by RefSeq, Jul 2008]

uniprot summary :

DKK1: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Interacts with LRP6. Interacts (via the C-termianl Cys- rich domain) with LRP5 (via beta-propeller regions 3 and 4); the interaction, enhanced by MESD and or KREMEN, antagonizes Wnt- mediated signaling. Placenta. Belongs to the dickkopf family. Protein type: Inhibitor; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 10q11.2. Cellular Component: extracellular space; early endosome membrane; plasma membrane; extracellular region. Molecular Function: protein binding; signal transducer activity; receptor antagonist activity; growth factor activity; low-density lipoprotein receptor binding. Biological Process: cellular morphogenesis during differentiation; negative regulation of skeletal muscle development; negative regulation of Wnt receptor signaling pathway; response to retinoic acid; regulation of receptor internalization; negative regulation of peptidyl-serine phosphorylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of BMP signaling pathway; mesoderm formation; negative regulation of mesodermal cell fate specification; hair follicle development; endoderm formation; forebrain development; regulation of endodermal cell fate specification; negative regulation of protein complex assembly; embryonic limb morphogenesis

size1 :

48-Strip-Wells

price1 :

410 USD

size2 :

96-Strip-Wells

price2 :

490

more info or order :

MyBioSource product webpage