ELISA/assay

Human BPI (Bactericidal/Permeability Increasing Protein) ELISA Kit

MBS2502375

24-Strip-Wells

215 USD

ELISA Kit

Human BPI (Bactericidal/Permeability Increasing Protein) ELISA Kit

[BPI]

[bactericidal permeability-increasing protein; Bactericidal permeability-increasing protein; bactericidal permeability-increasing protein; BPI fold containing family D, member 1; CAP 57; recombinant BPI holoprotein, rBPI; bactericidal/permeability-increasing protein; CAP 57]

[BPI]

[BPI; BPI; rBPI; BPIFD1; BPI]

BPI_HUMAN

Human

487

This kit recognizes natural and some recombinant Human BPI. No significant crossreactivity or interference between Human BPI and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL

Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Human BPI concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Human BPI. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Human BPI and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Human BPI, biotinylated detection antibody and Avidin- HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human BPI. The concentration of Human BPI in samples can be calculated by comparing the OD of the samples with the standard curve.

157276599

NP_001716.2

NM_001725.2

P17213

53,900 Da

This gene encodes a lipopolysaccharide binding protein. It is associated with human neutrophil granules and has antimicrobial activity against gram-negative organisms. [provided by RefSeq, Nov 2014]

BPI: The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family. Protein type: Cell surface; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 20q11.23. Cellular Component: integral to plasma membrane; cytoplasm. Molecular Function: lipopolysaccharide binding. Biological Process: negative regulation of interleukin-6 production; negative regulation of interleukin-8 production; immune response; negative regulation of tumor necrosis factor production; defense response to Gram-negative bacterium; negative regulation of macrophage activation

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590