Human VWFCP/ADAMTS13 (Von Willebrand Factor Cleaving Protease) ELISA Kit | MyBioSource MBS2502374 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human VWFCP/ADAMTS13 (Von Willebrand Factor Cleaving Protease) ELISA Kit

catalog :

MBS2502374

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2502374

products type :

ELISA Kit

products full name :

Human VWFCP/ADAMTS13 (Von Willebrand Factor Cleaving Protease) ELISA Kit

products short name :

[VWFCP/ADAMTS13]

other names :

[von Willebrand factor-cleaving protease; A disintegrin and metalloproteinase with thrombospondin motifs 13; A disintegrin and metalloproteinase with thrombospondin motifs 13; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 13; vWF-cleaving protease; von Willebrand factor-cleaving protease; ADAM metallopeptidase with thrombospondin type 1 motif, 13; von Willebrand factor-cleaving protease; vWF-CP; vWF-cleaving protease]

products gene name :

[VWFCP/ADAMTS13]

other gene names :

[ADAMTS13; ADAMTS13; VWFCP; C9orf8; vWF-CP; ADAM-TS13; ADAMTS-13; C9orf8; ADAM-TS 13; ADAM-TS13; ADAMTS-13; vWF-CP; vWF-cleaving protease]

uniprot entry name :

ATS13_HUMAN

reactivity :

Human

sequence length :

1427

specificity :

This kit recognizes natural and recombinantHumanVWFCP/ADAMTS13. No significant cross-reactivity or interference between HumanVWFCP/ADAMTS13 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL

products description :

Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanVWFCP/ADAMTS13concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to VWFCP/ADAMTS13. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for VWFCP/ADAMTS13and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain VWFCP/ADAMTS13, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of VWFCP/ADAMTS13.You can calculate the concentration of VWFCP/ADAMTS13in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

16306598

ncbi acc num :

AAL17652.1

ncbi mol weight :

39,864 Da

ncbi pathways :

Metabolism Of Proteins Pathway (106230); O-glycosylation Of TSR Domain-containing Proteins Pathway (1127680); O-linked Glycosylation Pathway (1127679); Post-translational Protein Modification Pathway (161012)

ncbi summary :

This gene encodes a member of a family of proteins containing several distinct regions, including a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. The enzyme encoded by this gene specifically cleaves von Willebrand Factor (vWF). Defects in this gene are associated with thrombotic thrombocytopenic purpura. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

uniprot summary :

ADAMTS13: Cleaves the vWF multimers in plasma into smaller forms. Defects in ADAMTS13 are the cause of thrombotic thrombocytopenic purpura congenital (TTP); also known as Upshaw-Schulman syndrome (USS). A hematologic disease characterized by hemolytic anemia with fragmentation of erythrocytes, thrombocytopenia, diffuse and non-focal neurologic findings, decreased renal function and fever. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Calcium-binding; Secreted; Secreted, signal peptide; Protease; Motility/polarity/chemotaxis; EC 3.4.24.87; Extracellular matrix. Chromosomal Location of Human Ortholog: 9q34. Cellular Component: extracellular space; proteinaceous extracellular matrix; cell surface; endoplasmic reticulum lumen. Molecular Function: integrin binding; protein binding; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity; calcium ion binding. Biological Process: integrin-mediated signaling pathway; protein amino acid O-linked glycosylation; platelet activation; cellular protein metabolic process; glycoprotein metabolic process; response to toxin; cell-matrix adhesion; peptide catabolic process; protein processing; post-translational protein modification; proteolysis. Disease: Thrombotic Thrombocytopenic Purpura, Congenital

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590