Mouse PACAP-27 (Pituitary Adenylate Cyclase Activating Polypeptide 27) ELISA Kit | MyBioSource MBS2502335 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse PACAP-27 (Pituitary Adenylate Cyclase Activating Polypeptide 27) ELISA Kit

catalog :

MBS2502335

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2502335

products type :

ELISA Kit

products full name :

Mouse PACAP-27 (Pituitary Adenylate Cyclase Activating Polypeptide 27) ELISA Kit

products short name :

[PACAP-27]

other names :

[pituitary adenylate cyclase-activating polypeptide type I receptor isoform 4; Pituitary adenylate cyclase-activating polypeptide type I receptor; pituitary adenylate cyclase-activating polypeptide type I receptor; PACAP receptor 1; PACAP type I receptor; PACAP-R1; pituitary adenylate cyclase activating polypeptide 1 receptor type I Hiphop; adenylate cyclase activating polypeptide 1 (pituitary) receptor type I]

products gene name :

[PACAP-27]

other gene names :

[ADCYAP1R1; ADCYAP1R1; PAC1; PAC1R; PACAPR; PACAPRI; PACAP type I receptor; PACAP-R-1; PACAP-R1]

uniprot entry name :

PACR_HUMAN

reactivity :

Mouse

sequence length :

447

specificity :

This kit recognizes natural and recombinant Mouse PACAP-27. No significant cross-reactivity or interference between Mouse PACAP-27 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse PACAP-27 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PACAP-27. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PACAP-27 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PACAP-27, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PACAP-27. You can calculate the concentration of PACAP-27 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

313851023

ncbi acc num :

NP_001186566.1

ncbi gb acc num :

NM_001199637.1

uniprot acc num :

P41586

ncbi mol weight :

46,957 Da

ncbi pathways :

Activation Of TRKA Receptors Pathway (106456); Circadian Entrainment Pathway (698773); Circadian Entrainment Pathway (699872); Class B/2 (Secretin Family Receptors) Pathway (106378); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class B Secretin-like Pathway (198781)

ncbi summary :

This gene encodes type I adenylate cyclase activating polypeptide receptor, which is a membrane-associated protein and shares significant homology with members of the glucagon/secretin receptor family. This receptor mediates diverse biological actions of adenylate cyclase activating polypeptide 1 and is positively coupled to adenylate cyclase. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Dec 2010]

uniprot summary :

ADCYAP1R1: This is a receptor for PACAP-27 and PACAP-38. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. May regulate the release of adrenocorticotropin, luteinizing hormone, growth hormone, prolactin, epinephrine, and catecholamine. May play a role in spermatogenesis and sperm motility. Causes smooth muscle relaxation and secretion in the gastrointestinal tract. Belongs to the G-protein coupled receptor 2 family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, multi-pass; GPCR, family 2; Receptor, GPCR; Membrane protein, integral. Chromosomal Location of Human Ortholog: 7p14. Cellular Component: neuron projection; cell surface; tight junction; rough endoplasmic reticulum; integral to plasma membrane; plasma membrane; caveola; receptor complex; endosome. Molecular Function: neuropeptide binding; ADP-ribosylation factor binding; vasoactive intestinal polypeptide receptor activity; protein binding; receptor activity; adenylate cyclase binding. Biological Process: response to drug; development of primary female sexual characteristics; nerve growth factor receptor signaling pathway; adenylate cyclase activation; response to estradiol stimulus; G-protein coupled receptor protein signaling pathway; cAMP-mediated signaling; cell surface receptor linked signal transduction; response to ethanol; phospholipase C activation; spermatogenesis; cell differentiation; multicellular organismal response to stress; transmembrane receptor protein tyrosine kinase signaling pathway

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590