catalog number :
MBS2502333
products type :
ELISA Kit
products full name :
Rabbit IL-8 (Interleukin 8) ELISA Kit
products short name :
[IL-8]
other names :
[interleukin-8; Interleukin-8; interleukin-8; C-X-C motif chemokine 8; IL-8; alveolar macrophage chemotactic factor I; alveolar macrophage-derived chemotactic factor-I; chemokine (C-X-C motif) ligand 8; Alveolar macrophage chemotactic factor I; AMCF-I; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8]
products gene name :
[IL-8]
other gene names :
[IL8; CXCL8; CXCL8; AMCF-I; IL8; IL-8; AMCF-I]
uniprot entry name :
IL8_PIG
specificity :
This kit recognizes natural and some recombinant Rabbit IL-8. No significant crossreactivity or interference between Rabbit IL-8 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL
products description :
Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Rabbit IL-8 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Rabbit IL-8. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Rabbit IL-8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Rabbit IL-8, biotinylated detection antibody and Avidin- HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit IL-8. The concentration of Rabbit IL-8 in samples can be calculated by comparing the OD of the samples with the standard curve.
ncbi acc num :
NP_999032.1
ncbi gb acc num :
NM_213867.1
ncbi mol weight :
11,633 Da
ncbi pathways :
Amoebiasis Pathway (167313); Amoebiasis Pathway (167191); Bladder Cancer Pathway (84522); Bladder Cancer Pathway (527); Cellular Senescence Pathway (1125456); Cellular Responses To Stress Pathway (1125444); Chagas Disease (American Trypanosomiasis) Pathway (147807); Chagas Disease (American Trypanosomiasis) Pathway (147795); Chemokine Receptors Bind Chemokines Pathway (1124657); Chemokine Signaling Pathway (98758)
uniprot summary :
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.
size5 :
10x96-Strip-Wells