catalog number :
MBS2502327
products type :
ELISA Kit
products full name :
Rat SHBG (Sex Hormone-Binding Globulin) ELISA Kit
products short name :
[SHBG]
other names :
[sex hormone-binding globulin isoform 1; Sex hormone-binding globulin; sex hormone-binding globulin; sex steroid-binding protein; testis-specific androgen-binding protein; testosterone-binding beta-globulin; testosterone-estradiol-binding globulin; testosterone-estrogen-binding globulin; sex hormone-binding globulin; Sex steroid-binding protein; SBP; Testis-specific androgen-binding protein; ABP; Testosterone-estradiol-binding globulin; TeBG; Testosterone-estrogen-binding globulin]
products gene name :
[SHBG]
other gene names :
[SHBG; SHBG; ABP; SBP; TEBG; SHBG; SBP; ABP; TeBG]
uniprot entry name :
SHBG_HUMAN
specificity :
This kit recognizes Rat sE-selectin in samples. No Significant cross-reactivity or interference between Rat sE-selectin and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 62.50-4000 pg/mL. Sensitivity: 37.50 pg/mL
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat sE-selectin were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat sE-selectin were tested on 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat sE-selectin concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat sE-selectin. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat sE-selectin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat sE-selectin, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat sE-selectin. You can calculate the concentration of Rat sE-selectin in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_001031.2
ncbi gb acc num :
NM_001040.4
ncbi mol weight :
41,712 Da
ncbi pathways :
Nongenotropic Androgen Signaling Pathway (138048)
ncbi summary :
This gene encodes a steroid binding protein that was first described as a plasma protein secreted by the liver but is now thought to participate in the regulation of steroid responses. The encoded protein transports androgens and estrogens in the blood, binding each steroid molecule as a dimer formed from identical or nearly identical monomers. Polymorphisms in this gene have been associated with polycystic ovary syndrome and type 2 diabetes mellitus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
uniprot summary :
SHBG: Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 17p13.1. Cellular Component: extracellular region. Molecular Function: androgen binding. Biological Process: primary spermatocyte growth
size5 :
10x96-Strip-Wells