ELISA/assay

Human Flt3L (FMS Like Tyrosine Kinase 3 Ligand) ELISA Kit

MBS2502306

24-Strip-Wells

215 USD

ELISA Kit

Human Flt3L (FMS Like Tyrosine Kinase 3 Ligand) ELISA Kit

[Flt3L]

[fms-related tyrosine kinase 3 ligand isoform 2; Fms-related tyrosine kinase 3 ligand; fms-related tyrosine kinase 3 ligand; flt3 ligand; fms-related tyrosine kinase 3 ligand; SL cytokine]

[Flt3L]

[FLT3LG; FLT3LG; FL; FLT3L; Flt3 ligand; Flt3L]

FLT3L_HUMAN

Human

153

This kit recognizes natural and recombinant Human Flt3L. No significant cross-reactivity or interference between Human Flt3L and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 15.625-1000pg/mL. Sensitivity: Min: 9.375pg/mL; Max: 1000pg/mL

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human Flt3L concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Flt3L. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Flt3L and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Flt3L, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Flt3L. You can calculate the concentration of Flt3L in the samples by comparing the OD of the samples to the standard curve.

520975474

NP_001265567.1

NM_001278638.1

P49771

17,290 Da

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Pathways In Cancer (83105)

Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3LG controls the development of DCs and is particularly important for plasmacytoid DCs and CD8 (see MIM 186910)-positive classical DCs and their CD103 (ITGAE; MIM 604682)-positive tissue counterparts (summary by Sathaliyawala et al., 2010 [PubMed 20933441]).[supplied by OMIM, Jan 2011]

FLT3LG: Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Cytokine; Ligand, receptor tyrosine kinase. Chromosomal Location of Human Ortholog: 19q13.3. Cellular Component: extracellular space; membrane; integral to membrane. Molecular Function: protein homodimerization activity; cytokine activity; receptor tyrosine kinase binding; receptor binding. Biological Process: positive regulation of natural killer cell differentiation; regulation of myeloid dendritic cell activation; lymphocyte differentiation; embryonic hemopoiesis; positive regulation of cell proliferation; positive regulation of protein amino acid phosphorylation; signal transduction; positive regulation of myoblast differentiation

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590