ELISA/assay

Human GAP43 (Growth Associated Protein 43) ELISA Kit

MBS2502209

24-Strip-Wells

215 USD

ELISA Kit

Human GAP43 (Growth Associated Protein 43) ELISA Kit

[GAP43]

[GAP43; Neuromodulin; Axonal membrane protein GAP-43; Calmodulin-binding protein P-57; Growth-associated protein 43]

[GAP43]

[GAP43]

NEUM_BOVIN

Human

242

This kit recognizes natural and recombinant Human GAP43. No significant cross-reactivity or interference between Human GAP43 and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human GAP43 concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GAP43. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GAP43 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GAP43, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of GAP43. You can calculate the concentration of GAP43 in the samples by comparing the OD of the samples to the standard curve.

37781182

AAO60065.1

25,066 Da

GAP43: This protein is associated with nerve growth. It is a major component of the motile growth cones that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction. Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence. Belongs to the neuromodulin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cell surface. Cellular Component: filopodium membrane; axon; postsynaptic density; cell junction. Molecular Function: calmodulin binding. Biological Process: glial cell differentiation; cell fate commitment; regulation of growth; axon choice point recognition; regulation of filopodium formation

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590