catalog number :
MBS2502190
products type :
ELISA Kit
products full name :
Rat FSH (Follicle Stimulating Hormone) ELISA Kit
products short name :
[FSH]
other names :
[FSH; Bromodomain-containing protein 2; O27.1.1; Really interesting new gene 3 protein]
products gene name :
[FSH]
other gene names :
[BRD2; KIAA9001; RING3]
uniprot entry name :
BRD2_HUMAN
specificity :
This kit recognizes Rat FLAP in samples. No significant cross-reactivity or interference between Rat FLAP and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 15.63-1000pg/mL. Sensitivity: 9.38pg/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat FLAP were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat FLAP were tested on 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat FLAP concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat FLAP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat FLAP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat FLAP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat FLAP. You can calculate the concentration of Rat FLAP in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
CAA43996.1
ncbi mol weight :
74,881 Da
uniprot summary :
BRD2: an atypical protein kinase with two bromodomains that bind to acetylated lysine residues. Specifically interacts with transcriptionally active chromatin. Elevated protein kinase activity in leukemias. Transgenic mice overexpressing BRD2 in the lymphoid system develop diffuse large-cell lymphoma. Interacts with E2F1 and with histone H4 acetylated at Lys-13. May play a role in spermatogenesis or folliculogenesis. Genetic evidence links the BRD2 gene to both juvenile myoclonic epilepsy and photoparoxysomal responses. Two isoforms of the human protein are produced by alternative splicing. Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, atypical; ATYPICAL group; BRD family. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: cytoplasm; nucleus. Molecular Function: protein binding; chromatin binding. Biological Process: regulation of transcription from RNA polymerase II promoter; nucleosome assembly; transcription, DNA-dependent; spermatogenesis; chromatin modification
size5 :
10x96-Strip-Wells