Porcine IL-12 (Interleukin 12) ELISA Kit | MyBioSource MBS2502160 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Porcine IL-12 (Interleukin 12) ELISA Kit

catalog :

MBS2502160

quantity :

24-Strip-Wells

price :

235 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2502160

products type :

ELISA Kit

products full name :

Porcine IL-12 (Interleukin 12) ELISA Kit

products short name :

[IL-12]

other names :

[Il-12 receptor beta2; Interleukin-12 receptor subunit beta-2; interleukin-12 receptor subunit beta-2; IL-12 receptor subunit beta-2; IL-12R subunit beta-2; interleukin-12 receptor beta-2 chain; interleukin 12 receptor, beta 2]

products gene name :

[IL-12]

other gene names :

[IL12RB2; IL12RB2; IL-12 receptor subunit beta-2; IL-12R subunit beta-2; IL-12R-beta-2; IL-12RB2]

uniprot entry name :

I12R2_HUMAN

reactivity :

Porcine

sequence length :

862

specificity :

This kit recognizes natural and recombinantPorcineIL-12. No significant cross-reactivity or interference between PorcineIL-12 and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL

products description :

Intended Uses: This ELISA kit applies to the invitro quantitative determination of PorcineIL-12concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IL-12. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-12and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IL-12.You can calculate the concentration of IL-12in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

1685028

ncbi acc num :

AAB36675.1

uniprot acc num :

Q99665

ncbi mol weight :

87,484 Da

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); IL12-mediated Signaling Events Pathway (137922); IL27-mediated Signaling Events Pathway (137929); Inflammatory Bowel Disease (IBD) Pathway (842771); Inflammatory Bowel Disease (IBD) Pathway (842797); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488)

ncbi summary :

The protein encoded by this gene is a type I transmembrane protein identified as a subunit of the interleukin 12 receptor complex. The coexpression of this and IL12RB1 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. The expression of this gene is up-regulated by interferon gamma in Th1 cells, and plays a role in Th1 cell differentiation. The up-regulation of this gene is found to be associated with a number of infectious diseases, such as Crohn's disease and leprosy, which is thought to contribute to the inflammatory response and host defense. Several transcript variants encoding different isoforms and non-protein coding transcripts have been found for this gene. [provided by RefSeq, Apr 2012]

uniprot summary :

IL12RB2: Receptor for interleukin-12. This subunit is the signaling component coupling to the JAK2/STAT4 pathway. Promotes the proliferation of T-cells as well as NK cells. Induces the promotion of T-cells towards the Th1 phenotype by strongly enhancing IFN-gamma production. Belongs to the type I cytokine receptor family. Type 2 subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cell cycle regulation; Receptor, cytokine; Membrane protein, integral. Chromosomal Location of Human Ortholog: 1p31.3-p31.2. Cellular Component: integral to plasma membrane; external side of plasma membrane. Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein kinase binding. Biological Process: positive regulation of interferon-gamma production; cell surface receptor linked signal transduction; peptidyl-tyrosine phosphorylation; cytokine and chemokine mediated signaling pathway; positive regulation of cell proliferation; interferon-gamma production; response to lipopolysaccharide

size1 :

24-Strip-Wells

price1 :

235 USD

size2 :

48-Strip-Wells

price2 :

450

size3 :

96-Strip-Wells

price3 :

535

size4 :

5x96-Strip-Wells

price4 :

2255

size5 :

10x96-Strip-Wells

price5 :

3975

more info or order :

MyBioSource product webpage