ELISA/assay

Human INSL3(Insulin Like Protein 3)ELISA Kit

MBS2502152

48-Strip-Wells

410 USD

ELISA Kit

Human INSL3(Insulin Like Protein 3)ELISA Kit

INSL3

insulin-like 3 isoform 1; Insulin-like 3; insulin-like 3; leydig insulin -like hormone; leydig insulin-like peptide; prepro-INSL3; relaxin-like factor b; insulin-like 3 (Leydig cell); Leydig insulin-like peptide; Ley-I-L; Relaxin-like factorCleaved into the following 2 chains:Insulin-like 3 B chain; Insulin-like 3 A chain

INSL3

INSL3; INSL3; RLF; RLNL; ley-I-L; RLF; RLNL; Ley-I-L

INSL3_HUMAN

Human

157

This assay has high sensitivity and excellent specificity for detection of Human INSL3. No significant cross-reactivity or interference between Human INSL3 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human INSL3 and all the analogues, therefore, cross reaction may still exist.

Store at 4 degree C.

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156--10ng/mL. Sensitivity: 0.094ng/mL

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of INSL3 in human serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human INSL3. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human INSL3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human INSL3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human INSL3. You can calculate the concentration of Human INSL3 in the samples by comparing the OD of the samples to the standard curve.

388240756

NP_001252516.1

NM_001265587.1

P51460

16,872 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Metabolic Disorders Of Biological Oxidation Enzymes Pathway (1127637); Peptide Ligand-binding Receptors Pathway (106358); Relaxin Receptors Pathway (161022); Signal Transduction Pathway (477114)

This gene encodes a member of the insulin-like hormone superfamily. The encoded protein is mainly produced in gonadal tissues. Studies of the mouse counterpart suggest that this gene may be involved in the development of urogenital tract and female fertility. This protein may also act as a hormone to regulate growth and differentiation of gubernaculum, and thus mediating intra-abdominal testicular descent. Mutations in this gene may lead to cryptorchidism. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2012]

INSL3: Seems to play a role in testicular function. May be a trophic hormone with a role in testicular descent in fetal life. Is a ligand for LGR8 receptor. Defects in INSL3 seems to be a cause of cryptorchidism (CRYPTO); also known as impaired testicular descent. It is one of the most frequent congenital abnormalities in humans, involving 2-5% of male births. Cryptorchidism is associated with increased risk of infertility and testicular cancer. The frequency of INSL3 gene mutations as a cause of cryptorchidism is low. Belongs to the insulin family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 19p13.2-p12. Cellular Component: extracellular space; perinuclear region of cytoplasm; extracellular region. Molecular Function: G-protein-coupled receptor binding; protease binding; hormone activity; insulin receptor binding; receptor binding. Biological Process: negative regulation of cell proliferation; cell-cell signaling; in utero embryonic development; positive regulation of cell proliferation; positive regulation of cAMP biosynthetic process; male gonad development; spermatogenesis; G-protein signaling, adenylate cyclase inhibiting pathway; oocyte maturation; negative regulation of apoptosis. Disease: Cryptorchidism, Unilateral Or Bilateral

48-Strip-Wells

410 USD

96-Strip-Wells

490