catalog number :
MBS2502115
products type :
ELISA Kit
products full name :
Human SCCA2 (Squamous Cell Carcinoma Antigen 2) ELISA Kit
products short name :
SCCA2
other names :
serpin B4 isoform 1; Serpin B4; serpin B4; SCCA2/SCCA1 fusion protein; peptidase inhibitor 11; protease inhibitor (leucine-serpin); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 4; squamous cell carcinoma antigen 1; squamous cell carcinoma antigen 2; serpin peptidase inhibitor, clade B (ovalbumin), member 4; Leupin; Peptidase inhibitor 11; PI-11; Squamous cell carcinoma antigen 2; SCCA-2
products gene name :
SCCA2
other gene names :
SERPINB4; SERPINB4; PI11; SCCA1; SCCA2; LEUPIN; SCCA-2; PI11; SCCA2; PI-11; SCCA-2
uniprot entry name :
SPB4_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of Human SCCA2. No significant cross-reactivity or interference between Human SCCA2 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human SCCA2 and all the analogues, therefore, cross reaction may still exist.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156--10ng/mL. Sensitivity: 0.094ng/mL
products description :
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SCCA2 in human serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SCCA2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human SCCA2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SCCA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human SCCA2. You can calculate the concentration of Human SCCA2 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_002965.1
ncbi gb acc num :
NM_002974.3
ncbi mol weight :
44,854 Da
ncbi pathways :
Amoebiasis Pathway (167324); Amoebiasis Pathway (167191)
uniprot summary :
SERPINB4: May act as a protease inhibitor to modulate the host immune response against tumor cells. Belongs to the serpin family. Ov-serpin subfamily. Protein type: Inhibitor. Chromosomal Location of Human Ortholog: 18q21.3. Cellular Component: extracellular space; cytoplasm; intracellular. Molecular Function: serine-type endopeptidase inhibitor activity; enzyme binding; protease binding. Biological Process: regulation of proteolysis; negative regulation of peptidase activity; protection from natural killer cell mediated cytotoxicity