catalog number :
MBS2501977
products type :
ELISA Kit
products full name :
Canine IgE (Immunoglobulin E) ELISA Kit
products short name :
IgE
other names :
immunoglobulin epsilon chain
specificity :
This kit recognizes natural and recombinant Canine IgE. No significant cross-reactivity or interference between Canine IgE and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 1.563-100ng/mL. Sensitivity: 0.938ng/mL
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Canine IgE concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IgE. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IgE and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IgE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of IgE. You can calculate the concentration of IgE in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
AAM45140.1