Rat COL2 (Collagen Type II) ELISA Kit | MyBioSource MBS2501969 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat COL2 (Collagen Type II) ELISA Kit

catalog :

MBS2501969

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2501969

products type :

ELISA Kit

products full name :

Rat COL2 (Collagen Type II) ELISA Kit

products short name :

[COL2]

other names :

[collagen alpha-1(II) chain isoform 1; Collagen alpha-1(II) chain; collagen alpha-1(II) chain; collagen alpha-1(II) chain; alpha-1 type II collagen; chondrocalcin; disproportionate micromelia; procollagen, type II, alpha 1; collagen, type II, alpha 1; Alpha-1 type II collagenCleaved into the following 2 chains:Collagen alpha-1(II) chain; Chondrocalcin]

products gene name :

[COL2]

other gene names :

[Col2a1; Col2a1; Dmm; Lpk; Col2; Del1; Col2a; Col2a-1; M100856; Rgsc856]

uniprot entry name :

CO2A1_MOUSE

reactivity :

Rat

sequence length :

1487

specificity :

This kit recognizes natural and some recombinant Rat COL2. No significant crossreactivity or interference between Rat COL2 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 62.5-4000pg/mL. Sensitivity: Min: 37.5pg/mL; Max: 4000pg/mL

products description :

Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Rat COL2 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Rat COL2. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Rat COL2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Rat COL2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat COL2. The concentration of Rat COL2 in samples can be calculated by comparing the OD of the samples with the standard curve.

ncbi gi num :

166064040

ncbi acc num :

NP_112440.2

ncbi gb acc num :

NM_031163.3

uniprot acc num :

P28481

ncbi mol weight :

134,722 Da

ncbi pathways :

Amoebiasis Pathway (167330); Amoebiasis Pathway (167191); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (1110305); Collagen Biosynthesis And Modifying Enzymes Pathway (1110304); Collagen Formation Pathway (1110303); ECM-receptor Interaction Pathway (83265); ECM-receptor Interaction Pathway (479); Endochondral Ossification Pathway (198336); Extracellular Matrix Organization Pathway (1110302); Focal Adhesion Pathway (198353)

uniprot summary :

COL2A1: the alpha-1 chain of type II collagen, an extra-cellular matrix protein found in cartilage and the vitreous humor of the eye. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Chondrocalcin is the calcium binding C-propeptide of this collagen molecule. Defects in this protein are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. There are two transcripts identified for this gene. Belongs to the fibrillar collagen family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Extracellular matrix; Secreted, signal peptide. Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; collagen; collagen type II; cytoplasm; extracellular region; basement membrane. Molecular Function: identical protein binding; platelet-derived growth factor binding; metal ion binding; extracellular matrix structural constituent. Biological Process: heart morphogenesis; proteoglycan metabolic process; inner ear morphogenesis; collagen fibril organization; palate development; limb morphogenesis; sensory perception of sound; visual perception; skeletal morphogenesis; regulation of gene expression; cartilage development; tissue homeostasis; chondrocyte differentiation; inner ear development; skeletal development; cartilage condensation; endochondral ossification

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590