Rat PSA (Prostate Specific Antigen) ELISA Kit | MyBioSource MBS2501876 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat PSA (Prostate Specific Antigen) ELISA Kit

catalog :

MBS2501876

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2501876

products type :

ELISA Kit

products full name :

Rat PSA (Prostate Specific Antigen) ELISA Kit

products short name :

[PSA]

other names :

[prostate-specific antigen isoform 1 preproprotein; Prostate-specific antigen; prostate-specific antigen; P-30 antigen; gamma-seminoprotein; kallikrein-3; prostate specific antigen; semenogelase; seminin; kallikrein-related peptidase 3; Gamma-seminoprotein; Seminin; Kallikrein-3; P-30 antigen; Semenogelase]

products gene name :

[PSA]

other gene names :

[KLK3; KLK3; APS; PSA; hK3; KLK2A1; APS; PSA; Seminin]

uniprot entry name :

KLK3_HUMAN

reactivity :

Rat

sequence length :

261

specificity :

This kit recognizes Rat PPAR-alpha in samples. No significant cross-reactivity or interference between Rat PPAR-alpha and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.31-20ng/mL. Sensitivity: 0.19ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat PPAR-alpha were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat PPAR-alpha were tested on 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat PPAR-alpha concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PPAR-alpha. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PPAR-alpha and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PPAR-alpha, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat PPAR-alpha. You can calculate the concentration of Rat PPAR-alpha in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

4502173

ncbi acc num :

NP_001639.1

ncbi gb acc num :

NM_001648.2

uniprot acc num :

P07288

ncbi mol weight :

25,116 Da

ncbi pathways :

Androgen Receptor Signaling Pathway (198806); Coregulation Of Androgen Receptor Activity Pathway (138085); FOXA1 Transcription Factor Network Pathway (137979); Metabolism Of Proteins Pathway (106230); Pathways In Cancer (83105); Prostate Cancer Pathway (755440); Prostate Cancer Pathway (83111); Prostate Cancer Pathway (523); Regulation Of Androgen Receptor Activity Pathway (138027); Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway (105908)

ncbi summary :

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

uniprot summary :

PSA: Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. Forms heterodimer with SERPINA5. Inhibited by SERPINA5. Activity is strongly inhibited by Zn2+, 100 times more abundant in semen than in serum. This inhibition is relieved by exposure to semenogelins, which are avid zinc binders. Belongs to the peptidase S1 family. Kallikrein subfamily. Protein type: Secreted, signal peptide; Secreted; EC 3.4.21.77; Protease. Chromosomal Location of Human Ortholog: 19q13.41. Cellular Component: extracellular region; nucleus. Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity. Biological Process: negative regulation of angiogenesis; cellular protein metabolic process; proteolysis

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590