ELISA/assay

Porcine S100B (S100 Calcium Binding Protein B) ELISA Kit

MBS2501779

24-Strip-Wells

210 USD

ELISA Kit

Porcine S100B (S100 Calcium Binding Protein B) ELISA Kit

[S100B]

[S100B; Protein S100-B; protein S100-B; S-100 calcium-binding protein, beta chain; S-100 protein subunit beta; S100 calcium-binding protein, beta (neural); S100 calcium binding protein B; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B]

[S100B]

[S100B; S100B; NEF; S100; S100-B; S100beta]

S100B_HUMAN

Porcine

92

This kit recognizes Porcine REG3gamma in samples. No significant cross-reactivity or interference between PorcineREG3gammaand analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 1.56-100ng/mL. Sensitivity: 0.94ng/mL.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Porcine REG3gamma concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine REG3gamma. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine REG3gamma and Avidin-Horseradish Peroxidase (HRP) conjugate are added successivelyto each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine REG3gamma, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Porcine REG3gamma. You can calculate the concentration of Porcine REG3gamma in the samples by comparing the OD of the samples to the standard curve.

49457242

CAG46920.1

P04271

10,713 Da

Activated TLR4 Signalling Pathway (106400); Advanced Glycosylation Endproduct Receptor Signaling Pathway (187092); Cytosolic Sensors Of Pathogen-associated DNA Pathway (576255); DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway (833822); Immune System Pathway (106386); Innate Immune System Pathway (106387); MyD88 Cascade Initiated On Plasma Membrane Pathway (205107); MyD88 Dependent Cascade Initiated On Endosome Pathway (187081); MyD88-independent Cascade Pathway (106401); MyD88:Mal Cascade Initiated On Plasma Membrane Pathway (106390)

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. [provided by RefSeq, Jul 2008]

S100B: Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. The S100B dimer binds two molecules of STK38. Interacts with AGER. The S100B dimer interacts with two molecules of CAPZA1. Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. Belongs to the S-101 family. Protein type: Calcium-binding. Chromosomal Location of Human Ortholog: 21q22.3. Cellular Component: ruffle; extracellular space; intracellular membrane-bound organelle; cell soma; perinuclear region of cytoplasm; cytoplasm; extracellular region; nucleus. Molecular Function: identical protein binding; RAGE receptor binding; protein binding; protein homodimerization activity; zinc ion binding; calcium ion binding; calcium-dependent protein binding; tau protein binding. Biological Process: central nervous system development; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of apoptosis; response to glucocorticoid stimulus; memory; regulation of cell shape; cell proliferation; learning and/or memory; astrocyte differentiation; axonogenesis; response to methylmercury; positive regulation of cell proliferation; innate immune response; regulation of neuronal synaptic plasticity

24-Strip-Wells

210 USD

48-Strip-Wells

400

96-Strip-Wells

475

5x96-Strip-Wells

1975

10x96-Strip-Wells

3480