catalog number :
MBS2501642
products type :
ELISA Kit
products full name :
Porcine BDNF (Brain Derived Neurotrophic Factor) ELISA Kit
products short name :
[BDNF]
other names :
[BDNF, partial; BDNF; BDNF]
products gene name :
[BDNF]
other gene names :
[BDNF]
uniprot entry name :
D0VPU4_ONCMY
specificity :
This kit recognizes Porcine AP13 in samples. No significant cross-reactivity or interference between Porcine AP13 and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 125-8000pg/mL. Sensitivity: 75pg/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Porcine AP13 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Porcine AP13 were tested on 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Porcine AP13 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Porcine AP13. During the reaction, Porcine AP13 in the sample or standard competes with a fixed amount of Porcine AP13 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Porcine AP13. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Porcine AP13 in the samples is then determined by comparing the OD of the samples to the standard curve.
ncbi acc num :
ACY54685.1
ncbi mol weight :
27,051 Da
size5 :
10x96-Strip-Wells