catalog number :
MBS2501522
products type :
ELISA Kit
products full name :
Human SULT1A1 (Sulfotransferase 1A1) ELISA Kit
products short name :
[SULT1A1]
other names :
[sulfotransferase 1A1 isoform b; Sulfotransferase 1A1; sulfotransferase 1A1; P-PST 1; aryl sulfotransferase 1; phenol-sulfating phenol sulfotransferase 1; thermostable phenol sulfotransferase1; ts-PST; sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1; Aryl sulfotransferase 1; HAST1/HAST2; Phenol sulfotransferase 1; Phenol-sulfating phenol sulfotransferase 1; P-PST 1; ST1A3; Thermostable phenol sulfotransferase; Ts-PST]
products gene name :
[SULT1A1]
other gene names :
[SULT1A1; SULT1A1; PST; STP; STP1; P-PST; ST1A1; ST1A3; TSPST1; HAST1/HAST2; STP; STP1; ST1A1; P-PST 1; Ts-PST]
uniprot entry name :
ST1A1_HUMAN
specificity :
This kit recognizes Human STAT3 in samples. No significant cross-reactivity or interference between HumanSTAT3 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.31-20ng/mL. Sensitivity: 0.19ng/mL.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human STAT3 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human STAT3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human STAT3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human STAT3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human STAT3. You can calculate the concentration of Human STAT3 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_803880.1
ncbi gb acc num :
NM_177536.3
ncbi mol weight :
25,388 Da
ncbi pathways :
Arylamine Metabolism Pathway (198830); Biological Oxidations Pathway (105698); Chemical Carcinogenesis Pathway (673221); Chemical Carcinogenesis Pathway (673237); Cytosolic Sulfonation Of Small Molecules Pathway (105724); Defective AHCY Causes Hypermethioninemia With S-adenosylhomocysteine Hydrolase Deficiency (HMAHCHD) Pathway (1127639); Defective GCLC Causes Hemolytic Anemia Due To Gamma-glutamylcysteine Synthetase Deficiency (HAGGSD) Pathway (1127658); Defective GGT1 Causes Glutathionuria (GLUTH) Pathway (1127659); Defective GSS Causes Glutathione Synthetase Deficiency (GSS Deficiency) Pathway (1127660); Defective MAT1A Causes Methionine Adenosyltransferase Deficiency (MATD) Pathway (1127663)
ncbi summary :
Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene encodes one of two phenol sulfotransferases with thermostable enzyme activity. Multiple alternatively spliced variants that encode two isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
uniprot summary :
SULT1A1: Sulfotransferase that utilizes 3 -phospho-5 -adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of catecholamines, phenolic drugs and neurotransmitters. Has also estrogen sulfotransferase activity. responsible for the sulfonation and activation of minoxidil. Is Mediates the metabolic activation of carcinogenic N- hydroxyarylamines to DNA binding products and could so participate as modulating factor of cancer risk. Belongs to the sulfotransferase 1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Transferase; EC 2.8.2.1; Energy Metabolism - sulfur. Chromosomal Location of Human Ortholog: 16p12.1. Cellular Component: cytosol. Molecular Function: sulfotransferase activity; steroid sulfotransferase activity; flavonol 3-sulfotransferase activity; aryl sulfotransferase activity. Biological Process: estrogen metabolic process; flavonoid metabolic process; xenobiotic metabolic process; sulfation; catecholamine metabolic process; amine metabolic process; 3 -phosphoadenosine 5 -phosphosulfate metabolic process
size5 :
10x96-Strip-Wells