catalog number :
MBS2501506
products type :
ELISA Kit
products full name :
Rat EGF (Epidermal Growth Factor) ELISA Kit
products short name :
[EGF]
other names :
[epidermal growth factor, partial; Epidermal growth factor; Epidermal growth factor]
products gene name :
[EGF]
other gene names :
[epidermal growth factor/ EGF]
uniprot entry name :
Q28867_HORSE
specificity :
This kit recognizes Rat DPD in samples. No significant cross-reactivity or interference between Rat DPD and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 3.13-200ng/mL. Sensitivity: 1.88ng/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat DPD were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat DPD were tested on 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat DPD concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat DPD. During the reaction, Rat DPD in the sample or standard competes with a fixed amount of Rat DPD on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat DPD. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Rat DPD in the samples is then determined by comparing the OD of the samples to the standard curve.
ncbi acc num :
AAB32226.1
ncbi mol weight :
9,879 Da
size5 :
10x96-Strip-Wells