Rat OxLDL (Oxidized Lowdensity Lipoprotein) ELISA Kit | MyBioSource MBS2501477 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat OxLDL (Oxidized Lowdensity Lipoprotein) ELISA Kit

catalog :

MBS2501477

quantity :

24-Strip-Wells

price :

190 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2501477

products type :

ELISA Kit

products full name :

Rat OxLDL (Oxidized Lowdensity Lipoprotein) ELISA Kit

products short name :

[OxLDL]

other names :

[oxidized low-density lipoprotein receptor 1; Oxidized low-density lipoprotein receptor 1; oxidized low-density lipoprotein receptor 1; BLOX1; LOX-1; bLOX-1; lectin-like oxLDL receptor 1; lectin-like oxidized LDL receptor 1; lectin-type oxidized LDL receptor 1; ox-LDL receptor 1; oxidised low density lipoprotein (lectin-like) receptor 1; oxidized low density lipoprotein (lectin-like) receptor 1<; Lectin-like oxidized LDL receptor 1; LOX-1; Lectin-like oxLDL receptor 1; bLOX-1; Lectin-type oxidized LDL receptor 1Cleaved into the following 2 chains:Oxidized low-density lipoprotein receptor 1, soluble form A; Oxidized low-density lipoprotein receptor 1, soluble form B]

products gene name :

[OxLDL]

other gene names :

[OLR1; OLR1; LOX1; Ox-LDL receptor 1; LOX-1; Lectin-like oxLDL receptor 1; bLOX-1]

uniprot entry name :

OLR1_BOVIN

reactivity :

Rat

sequence length :

270

specificity :

This kit recognizes Rat OPG in samples. No significant cross-reactivity or interference between Rat OPG and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 62.5-4000pg/mL. Sensitivity: 37.5pg/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat OPG were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat OPG were tested on 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat OPG concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat OPG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat OPG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat OPG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat OPG. You can calculate the concentration of Rat OPG in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

27806503

ncbi acc num :

NP_776557.1

ncbi gb acc num :

NM_174132.2

uniprot acc num :

P79391

ncbi mol weight :

30,892 Da

ncbi pathways :

Cell Surface Interactions At The Vascular Wall Pathway (1120420); Hemostasis Pathway (1120392); PPAR Signaling Pathway (84281); PPAR Signaling Pathway (450); Phagosome Pathway (153904); Phagosome Pathway (153859)

uniprot summary :

Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria.

size1 :

24-Strip-Wells

price1 :

190 USD

size2 :

48-Strip-Wells

price2 :

310

size3 :

96-Strip-Wells

price3 :

355

size4 :

5x96-Strip-Wells

price4 :

1435

size5 :

10x96-Strip-Wells

price5 :

2805

more info or order :

MyBioSource product webpage