catalog number :
MBS2501454
products type :
ELISA Kit
products full name :
Rat VASPIN (Visceral Adipose Specific Serine Protease Inhibitor) ELISA Kit
products short name :
[VASPIN]
other names :
[visceral adipose tissue specific SERPIN; Serpin A12; Visceral adipose tissue-derived serine protease inhibitor; Vaspin; Visceral adipose-specific serpin]
products gene name :
[vaspin]
other gene names :
[Serpina12; Vaspin]
uniprot entry name :
SPA12_RAT
specificity :
This kit recognizes Rat TSH in samples. No significant cross-reactivity or interference between Rat TSH and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 1.25-80ng/mL. Sensitivity: 0.75ng/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat TSH were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat TSH were tested on 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat TSH concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TSH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TSH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TSH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat TSH. You can calculate the concentration of Rat TSH in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
AAL99574.1
ncbi mol weight :
47,527 Da
uniprot summary :
SERPINA12: May modulate insulin action conceivably only in the presence of its yet undefined target proteases in white adipose tissues. Belongs to the serpin family. Protein type: Secreted, signal peptide; Secreted; Inhibitor. Cellular Component: extracellular space; plasma membrane. Molecular Function: serine-type endopeptidase inhibitor activity. Biological Process: positive regulation of phosphoinositide 3-kinase cascade; negative regulation of lipid biosynthetic process; negative regulation of gluconeogenesis; glucose metabolic process; positive regulation of insulin receptor signaling pathway
size5 :
10x96-Strip-Wells