Human GDF3 (Growth Differentiation Factor 3) ELISA Kit | MyBioSource MBS2501371 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human GDF3 (Growth Differentiation Factor 3) ELISA Kit

catalog :

MBS2501371

quantity :

24-Strip-Wells

price :

215 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2501371

products type :

ELISA Kit

products full name :

Human GDF3 (Growth Differentiation Factor 3) ELISA Kit

products short name :

[GDF3]

other names :

[growth/differentiation factor 3; Growth/differentiation factor 3; growth/differentiation factor 3; GDF-3; growth differentiation factor 3]

products gene name :

[GDF3]

other gene names :

[GDF3; GDF3; KFS3; MCOP7; MCOPCB6; GDF-3]

uniprot entry name :

GDF3_HUMAN

reactivity :

Human

sequence length :

364

specificity :

This kit recognizes Human GDF11 in samples. No significant cross-reactivity or interference between HumanGDF11and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 15.63-1000pg/mL. Sensitivity: 9.38pg/mL.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human GDF11 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GDF11. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GDF11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GDF11, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human GDF11. You can calculate the concentration of Human GDF11 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

10190670

ncbi acc num :

NP_065685.1

ncbi gb acc num :

NM_020634.1

uniprot acc num :

Q9NR23

ncbi mol weight :

41,387 Da

ncbi summary :

The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. [provided by RefSeq, Jul 2008]

uniprot summary :

GDF3: Defects in GDF3 are the cause of Klippel-Feil syndrome type 3 (KFS3); also called Klippel-Feil syndrome autosomal dominant 3. KFS3 is a skeletal disorder characterized by congenital fusion of cervical vertebrae. It is due to a failure in the normal segmentation of vertebrae during the early weeks of fetal development. The clinical triad consists of short neck, low posterior hairline, and limited neck movement. Defects in GDF3 are the cause of microphthalmia isolated with coloboma type 6 (MCOPCB6); also called isolated colobomatous microphthalmia 6. MCOPCB6 is a disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataract and other abnormalities like cataract may also be present. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure). Defects in GDF3 are a cause of microphthalmia isolated type 7 (MCOP7). MCOP7 is a disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataract and other abnormalities like cataract may also be present. Belongs to the TGF-beta family. Protein type: Secreted, signal peptide; Secreted; Cytokine. Chromosomal Location of Human Ortholog: 12p13.1. Cellular Component: extracellular space; cytoplasm. Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding; protein kinase binding. Biological Process: response to dietary excess; in utero embryonic development; notochord development; somite rostral/caudal axis specification; formation of anatomical boundary; negative regulation of BMP signaling pathway; regulation of apoptosis; eye development; regulation of MAPKKK cascade; negative regulation of epidermal cell differentiation; regulation of cell fate commitment; mesoderm development; negative regulation of myoblast differentiation; cell development; skeletal development; endoderm development; growth. Disease: Microphthalmia, Isolated 7; Klippel-feil Syndrome 3, Autosomal Dominant; Microphthalmia, Isolated, With Coloboma 6

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590

more info or order :

MyBioSource product webpage