ELISA/assay

Mouse PPAR-gamma (Peroxisome Proliferator-activated receptor gamma) ELISA Kit

MBS2501353

48-Strip-Wells

410 USD

ELISA Kit

Mouse PPAR-gamma (Peroxisome Proliferator-activated receptor gamma) ELISA Kit

PPAR-gamma

peroxisome proliferator-activated receptor gamma isoform 1; Peroxisome proliferator-activated receptor gamma; peroxisome proliferator-activated receptor gamma; PPAR-gamma; nuclear receptor subfamily 1 group C member 3; peroxisome proliferator-activated nuclear receptor gamma variant 1; peroxisome proliferator-activated receptor gamma; Nuclear receptor subfamily 1 group C member 3

PPAR-gamma

PPARG; PPARG; GLM1; CIMT1; NR1C3; PPARG1; PPARG2; PPARgamma; NR1C3; PPAR-gamma

PPARG_HUMAN

Mouse

477

This kit recognizes natural and recombinant Mouse PPAR-gamma. No significant cross-reactivity or interference between Mouse PPAR-gamma and analogues was observed.

Store at 4 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: The minimum detectable dose of Mouse PPAR-gamma is 0.094ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse PPAR-gamma concentrations in serum, plasma and other biological fluids.

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PPAR-gamma. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PPAR-gamma and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PPAR-gamma, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PPAR-gamma. You can calculate the concentration of PPAR-gamma in the samples by comparing the OD of the samples to the standard curve.

116284370

NP_619726.2

NM_138712.3

P37231

21,580 Da

AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Adipogenesis Pathway (198832); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Developmental Biology Pathway (477129); Energy Metabolism Pathway (198907); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Huntington's Disease Pathway (83100)

This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008]

PPAR-gamma: a transcription factor, member of the nuclear hormone receptor superfamily. Receptor for hypolipidemic drugs and fatty acids. Preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage. Regulator of adipogenesis and lipid metabolism, modulates insulin sensitivity, cell proliferation and inflammation. Phosphorylated and inhibited by MAP kinase. Heterodimerizes with the retinoid X receptor. Interacts with NCOA6 coactivator, leading to a strong increase in transcription of target genes. Two splice-variant isoforms have been described. Protein type: Nuclear receptor; DNA-binding. Chromosomal Location of Human Ortholog: 3p25. Cellular Component: nucleoplasm; perinuclear region of cytoplasm; nucleus; cytosol. Molecular Function: ligand-dependent nuclear receptor activity; transcription activator binding; zinc ion binding; drug binding; alpha-actinin binding; protein phosphatase binding; arachidonic acid binding; retinoid X receptor binding; protein binding; enzyme binding; DNA binding; ligand-dependent nuclear receptor transcription coactivator activity; prostaglandin receptor activity; sequence-specific DNA binding; steroid hormone receptor activity; estrogen receptor binding; chromatin binding; transcription factor activity. Biological Process: negative regulation of smooth muscle cell proliferation; positive regulation of transcription, DNA-dependent; negative regulation of collagen biosynthetic process; heart development; low-density lipoprotein receptor biosynthetic process; cell maturation; rhythmic process; lipid homeostasis; negative regulation of transcription from RNA polymerase II promoter; response to lipid; glucose homeostasis; signal transduction; response to caffeine; response to vitamin A; epithelial cell differentiation; regulation of blood pressure; positive regulation of oligodendrocyte differentiation; response to nutrient; placenta development; caspase activation; long-chain fatty acid transport; transcription initiation from RNA polymerase II promoter; organ regeneration; response to retinoic acid; cell fate commitment; monocyte differentiation; negative regulation of acute inflammatory response; regulation of circadian rhythm; response to starvation; G-protein coupled receptor protein signaling pathway; negative regulation of telomerase activity; cellular response to insulin stimulus; response to estrogen stimulus; lipoprotein transport; response to mechanical stimulus; response to low density lipoprotein stimulus; positive regulation of fatty acid oxidation; white fat cell differentiation; brown fat cell differentiation; innate immune response; positive regulation of fat cell differentiation; steroid hormone mediated signaling; positive regulation of transcription factor activity; fatty acid oxidation; gene expression; positive regulation of transcription from RNA polymerase II promoter; lipid metabolic process; negative regulation of cell growth; response to cold; positive regulation of phagocytosis, engulfment; negative regulation of transcription, DNA-dependent. Disease: Obesity; Carotid Intimal Medial Thickness 1; Lipodystrophy, Familial Partial, Type 3; Diabetes Mellitus, Noninsulin-dependent

48-Strip-Wells

410 USD

96-Strip-Wells

490