ELISA/assay

Human TGF-beta1 (Transforming Growth Factor Beta 1) ELISA Kit

MBS2501101

24-Strip-Wells

190 USD

ELISA Kit

Human TGF-beta1 (Transforming Growth Factor Beta 1) ELISA Kit

[TGF-beta1]

[transforming growth factor beta-1; Transforming growth factor beta-1; transforming growth factor beta-1; TGF-beta 1; TGF-beta-1; regulatory protein; transforming growth factor-beta 1; transforming growth factor, beta 1]

[TGF-beta1]

[Tgfb1; Tgfb1; Tgfb; Tgfb-1; TGFbeta1; TGF-beta1; TGF-beta-1; LAP]

TGFB1_MOUSE

Human

390

This kit recognizes natural and some recombinant Human TGF -?1. No Significant cross -reactivity or interference between HumanTGF -?1 and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human TGF-beta1 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TGF-beta1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TGF-beta1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TGF-beta1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of TGF-beta1. You can calculate the concentration of TGF-beta1 in the samples by comparing the OD of the samples to the standard curve.

6755775

NP_035707.1

NM_011577.1

P04202

44,310 Da

Adipogenesis Pathway (198299); Amoebiasis Pathway (167330); Amoebiasis Pathway (167191); Cell Cycle Pathway (83251); Cell Cycle Pathway (198407); Cell Cycle Pathway (463); Cell Cycle Signaling Pathway (522974); Chagas Disease (American Trypanosomiasis) Pathway (147810); Chagas Disease (American Trypanosomiasis) Pathway (147795); Chronic Myeloid Leukemia Pathway (83309)

TGFB1: Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Homodimer; disulfide-linked, or heterodimer with TGFB2. Secreted and stored as a biologically inactive form in the extracellular matrix in a 290 kDa complex (large latent TGF-beta1 complex) containing the TGFB1 homodimer, the latency-associated peptide (LAP), and the latent TGFB1 binding protein-1 (LTBP1). The complex without LTBP1 is known as the small latent TGF-beta1 complex . Dissociation of the TGFB1 from LAP is required for growth factor activation and biological activity. Release of the large latent TGF-beta1 complex from the extracellular matrix is carried out by the matrix metalloproteinase MMP3. May interact with THSD4; this interaction may lead to sequestration by FBN1 microfibril assembly and attenuation of TGFB signaling. Interacts with the serine proteases, HTRA1 and HTRA3: the interaction with either inhibits TGFB1-mediated signaling. The HTRA protease activity is required for this inhibition. Interacts with CD109, DPT and ASPN. Activated in vitro at pH below 3.5 and over 12.5. Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage. Belongs to the TGF-beta family. Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide. Cellular Component: extracellular space; proteinaceous extracellular matrix; microvillus; cell surface; cell soma; axon; cell; cytoplasm; extracellular region; nucleus; secretory granule. Molecular Function: protein binding; protein homodimerization activity; enzyme binding; growth factor activity; protein heterodimerization activity; punt binding; cytokine activity; protein N-terminus binding; glycoprotein binding; transforming growth factor beta receptor binding; antigen binding. Biological Process: positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; SMAD protein nuclear translocation; positive regulation of protein amino acid dephosphorylation; activation of NF-kappaB transcription factor; regulation of protein import into nucleus; positive regulation of MAP kinase activity; regulation of transforming growth factor beta receptor signaling pathway; negative regulation of ossification; cell cycle arrest; positive regulation of isotype switching to IgA isotypes; T cell differentiation; regulatory T cell differentiation; positive regulation of interleukin-17 production; regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation; positive regulation of smooth muscle cell differentiation; positive regulation of chemotaxis; negative regulation of immune response; positive regulation of blood vessel endothelial cell migration; regulation of sodium ion transport; negative regulation of fat cell differentiation; negative regulation of blood vessel endothelial cell migration; lymph node development; positive regulation of protein secretion; positive regulation of cell division; regulation of MAPKKK cascade; positive regulation of transcription from RNA polymerase II promoter; response to progesterone stimulus; endoderm development; positive regulation of odontogenesis; myelination; negative regulation of phagocytosis; evasion of host defenses by virus; wound healing; T cell activation; positive regulation of cellular protein metabolic process; myeloid dendritic cell differentiation; negative regulation of transcription from RNA polymerase II promoter; phosphate metabolic process; response to organic substance; negative regulation of cell proliferation; CD4-positive, CD25-positive, alpha-beta regulatory T cell lineage commitment; negative regulation of T cell proliferation; mammary gland development; regulation of DNA binding; negative regulation of release of sequestered calcium ion into cytosol; positive regulation of cell proliferation; protein kinase B signaling cascade; protein export from nucleus; inflammatory response; positive regulation of exit from mitosis; epidermal growth factor receptor signaling pathway; mitotic cell cycle checkpoint; common-partner SMAD protein phosphorylation; positive regulation of phosphoinositide 3-kinase activity; positive regulation of peptidyl-serine phosphorylation; SMAD protein complex assembly; regulation of cell proliferation; positive regulation of protein kinase B signaling cascade; cell proliferation; negative regulation of interleukin-17 production; positive regulation of protein complex assembly; positive regulation of protein import into nucleus; epithelial to mesenchymal transition; negative regulation of cell growth; negative regulation of cell-cell adhesion; negative regulation of skeletal muscle development; mononuclear cell proliferation; protein amino acid phosphorylation; hyaluronan catabolic process; regulation of apoptosis; negative regulation of neuroblast proliferation; transforming growth factor beta receptor signaling pathway; positive regulation of superoxide release; receptor catabolic process; germ cell migration; chondrocyte differentiation; T cell homeostasis; negative regulation of mitotic cell cycle; defense response to fungus, incompatible interaction; cell growth; tolerance induction to self antigen; regulation of striated muscle development; skeletal muscle development; organ regeneration; cell activation; organ morphogenesis; negative regulation of DNA replication; hemopoietic progenitor cell differentiation; negative regulation of transcription, DNA-dependent; positive regulation of epithelial cell proliferation; positive regulation of collagen biosynthetic process; defense response; response to estradiol stimulus; negative regulation of cell cycle; regulation of interleukin-23 production; positive regulation of histone deacetylation; negative regulation of protein amino acid phosphorylation; lipopolysaccharide-mediated signaling pathway; adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains; skeletal development; negative regulation of epithelial cell proliferation; intercellular junction assembly and maintenance; regulation of binding; MAPKKK cascade; morphogenesis of a branching structure; cellular calcium ion homeostasis; protein import into nucleus, translocation; ATP biosynthetic process; positive regulation of histone acetylation; positive regulation of protein amino acid phosphorylation; negative regulation of myoblast differentiation; negative regulation of T cell activation; positive regulation of cell migration; growth

24-Strip-Wells

190 USD

48-Strip-Wells

310

96-Strip-Wells

355

5x96-Strip-Wells

1435

10x96-Strip-Wells

2805