Rat DAO (Diamine Oxidase) ELISA Kit | MyBioSource MBS2501081 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat DAO (Diamine Oxidase) ELISA Kit

catalog :

MBS2501081

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2501081

products type :

ELISA Kit

products full name :

Rat DAO (Diamine Oxidase) ELISA Kit

products short name :

[DAO]

other names :

[D-amino-acid oxidase; D-amino-acid oxidase; D-amino-acid oxidase; D-amino acid oxidase; D-amino-acid oxidase]

products gene name :

[DAO]

other gene names :

[DAO; DAO; DAAO; OXDA; DAMOX; DAMOX; DAAO; DAMOX; DAO]

uniprot entry name :

OXDA_HUMAN

reactivity :

Rat

sequence length :

347

specificity :

This kit recognizes natural and recombinant Rat DAO. No significant cross-reactivity or interference between Rat DAO and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 3.125-200ng/mL. Sensitivity: Min: 1.875ng/mL; Max: 200ng/mL

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat DAO concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to DAO. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for DAO and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain DAO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of DAO. You can calculate the concentration of DAO in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

25453448

ncbi acc num :

P14920.3

uniprot acc num :

P14920

ncbi mol weight :

39,474 Da

ncbi pathways :

Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); D-Arginine And D-ornithine Metabolism Pathway (82972); D-Arginine And D-ornithine Metabolism Pathway (341); Glycine, Serine And Threonine Metabolism Pathway (82949); Glycine, Serine And Threonine Metabolism Pathway (313); Glyoxylate Metabolism Pathway (160990); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169)

ncbi summary :

This gene encodes the peroxisomal enzyme D-amino acid oxidase. The enzyme is a flavoprotein which uses flavin adenine dinucleotide (FAD) as its prosthetic group. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. Its biological function is not known; it may act as a detoxifying agent which removes D-amino acids that accumulate during aging. In mice, it degrades D-serine, a co-agonist of the NMDA receptor. This gene may play a role in the pathophysiology of schizophrenia. [provided by RefSeq, Jul 2008]

uniprot summary :

DAO: Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D- amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids. Belongs to the DAMOX/DASOX family. Protein type: Amino Acid Metabolism - glycine, serine and threonine; Other Amino Acids Metabolism - D-Arginine and D-ornithine; Oxidoreductase; Amino Acid Metabolism - arginine and proline; EC 1.4.3.3. Chromosomal Location of Human Ortholog: 12q24. Cellular Component: peroxisomal membrane; peroxisomal matrix; mitochondrial outer membrane; peroxisome; cytosol. Molecular Function: protein dimerization activity; protein binding; cofactor binding; D-amino-acid oxidase activity; receptor binding. Biological Process: proline catabolic process; glyoxylate metabolic process; dopamine biosynthetic process; leucine metabolic process. Disease: Schizophrenia

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590