catalog number :
MBS2501073
products type :
ELISA Kit
products full name :
Mouse OVA sIgE(Ovalbumin Specific IgE)ELISA Kit
products short name :
OVA sIgE
products gene name :
OVA sIgE
specificity :
This kit recognizes natural and recombinant Mouse OVA sIgE. No significant cross-reactivity or interference between Mouse OVA sIgE and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Detection Range: 1.563-100ng/mL. Sensitivity: The minimum detectable dose of Mouse OVA sIgE is 0.938ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero). Assay Type: Sandwich
other info2 :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse OVA sIgE concentrations in serum, plasma and other biological fluids.
products description :
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to OVA sIgE. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for OVA sIgE and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain OVA sIgE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of OVA sIgE. You can calculate the concentration of OVA sIgE in the samples by comparing the OD of the samples to the standard curve.