ELISA/assay

Monkey TSP-1 (Thrombospondin-1) ELISA Kit

MBS2501072

48-Strip-Wells

450 USD

ELISA Kit

Monkey TSP-1 (Thrombospondin-1) ELISA Kit

TSP-1

thrombospondin-1; Thrombospondin-1; thrombospondin-1; thrombospondin-1p180; thrombospondin 1

TSP-1

THBS1; THBS1; TSP; THBS; TSP1; TSP-1; THBS-1; TSP; TSP1

TSP1_HUMAN

Monkey

1170

This kit recognizes natural and recombinant Monkey TSP-1. No significant cross-reactivity or interference between Monkey TSP-1 and analogues was observed.

Store at 4 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 6.25-400ng/mL. Sensitivity: The minimum detectable dose of Monkey TSP-1 is 3.75ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Monkey TSP-1 concentrations in serum, plasma and other biological fluids.

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TSP-1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TSP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TSP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TSP-1. You can calculate the concentration of TSP-1 in the samples by comparing the OD of the samples to the standard curve.

40317626

NP_003237.2

NM_003246.2

P07996

120,148 Da

Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (576262); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); Hemostasis Pathway (106028); Inflammatory Response Pathway (198766)

The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. [provided by RefSeq, Jul 2008]

THBS1: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp. Ligand for CD36 mediating antiangiogenic properties. Belongs to the thrombospondin family. Protein type: Inhibitor; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 15q15. Cellular Component: extracellular matrix; extracellular space; cell surface; sarcoplasmic reticulum; endoplasmic reticulum lumen; endoplasmic reticulum; fibrinogen complex; extracellular region; external side of plasma membrane; secretory granule. Molecular Function: heparin binding; identical protein binding; laminin binding; calcium ion binding; integrin binding; proteoglycan binding; protein binding; fibroblast growth factor binding; transforming growth factor beta binding; phosphatidylserine binding; fibronectin binding; low-density lipoprotein binding; glycoprotein binding. Biological Process: extracellular matrix organization and biogenesis; activation of MAPK activity; response to magnesium ion; negative regulation of fibrinolysis; response to glucose stimulus; cell cycle arrest; cell adhesion; positive regulation of macrophage activation; response to drug; platelet activation; negative regulation of interleukin-12 production; positive regulation of chemotaxis; positive regulation of blood vessel endothelial cell migration; response to testosterone stimulus; negative regulation of cell-matrix adhesion; negative regulation of blood vessel endothelial cell migration; response to unfolded protein; positive regulation of angiogenesis; response to mechanical stimulus; negative regulation of endothelial cell proliferation; regulation of cGMP metabolic process; peptide cross-linking; response to calcium ion; response to progesterone stimulus; negative regulation of apoptosis; positive regulation of blood coagulation; positive regulation of translation; negative regulation of fibroblast growth factor receptor signaling pathway; negative regulation of antigen processing and presentation of peptide or polysaccharide antigen via MHC class II; negative regulation of caspase activity; behavioral response to pain; platelet degranulation; positive regulation of tumor necrosis factor biosynthetic process; protein amino acid O-linked glycosylation; positive regulation of transforming growth factor-beta1 production; cell migration; chronic inflammatory response; negative regulation of focal adhesion formation; positive regulation of transforming growth factor beta receptor signaling pathway; engulfment of apoptotic cell; post-translational protein modification; positive regulation of protein kinase B signaling cascade; negative regulation of angiogenesis; cellular protein metabolic process; negative regulation of dendritic cell antigen processing and presentation; response to hypoxia; immune response; sprouting angiogenesis; blood coagulation; positive regulation of phosphorylation; positive regulation of cell migration

48-Strip-Wells

450 USD

96-Strip-Wells

535