catalog number :
MBS2501041
products type :
ELISA Kit
products full name :
Rat IL-18 (Interleukin 18) ELISA Kit
products short name :
[IL-18]
other names :
[interleukin-18; Interleukin-18; interleukin-18; IFN-gamma-inducing factor; IL-1 gamma; IL-18; interferon gamma-inducing factor; interferon-gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); interleukin-1 gamma; interleukin 18<; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma]
products gene name :
[IL-18]
other gene names :
[IL18; IL18; IGIF; IL-18; IFN-gamma-inducing factor; IL-1 gamma]
uniprot entry name :
IL18_BOVIN
specificity :
This kit recognizes natural and some recombinant Rat IL-18 . No significant crossreactivity or interference between Rat IL-18 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 15.625-1000pg/mL. Sensitivity: Min: 9.375pg/mL; Max: 1000pg/mL
products description :
Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Rat IL-18 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Rat IL-18 . Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Rat IL-18 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Rat IL-18 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-18 . The concentration of Rat IL-18 in samples can be calculated by comparing the OD of the samples with the standard curve.
ncbi acc num :
NP_776516.1
ncbi gb acc num :
NM_174091.2
ncbi mol weight :
22,347 Da
ncbi pathways :
African Trypanosomiasis Pathway (194381); African Trypanosomiasis Pathway (194323); Cytokine Signaling In Immune System Pathway (1119410); Cytokine-cytokine Receptor Interaction Pathway (84290); Cytokine-cytokine Receptor Interaction Pathway (460); Cytosolic DNA-sensing Pathway (125127); Cytosolic DNA-sensing Pathway (124833); Hypertrophy Model Pathway (920905); Immune System Pathway (1119286); Inflammatory Bowel Disease (IBD) Pathway (842773)
uniprot summary :
Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
size5 :
10x96-Strip-Wells