Rat HMGB-1 (High Mobility Group Protein B1) ELISA Kit | MyBioSource MBS2500937 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat HMGB-1 (High Mobility Group Protein B1) ELISA Kit

catalog :

MBS2500937

quantity :

48-Strip-Wells

price :

410 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2500937

products type :

ELISA Kit

products full name :

Rat HMGB-1 (High Mobility Group Protein B1) ELISA Kit

products short name :

HMGB-1

other names :

high mobility group protein B1; High mobility group protein B1; high mobility group protein B1; HMG-1; high mobility group protein 1; high-mobility group box 1; high mobility group box 1<; High mobility group protein 1; HMG-1

products gene name :

HMGB-1

other gene names :

HMGB1; HMGB1; HMG1; HMG-1

uniprot entry name :

HMGB1_BOVIN

reactivity :

Rat

sequence length :

215

specificity :

This kit recognizes natural and recombinant Rat HMGB-1. No significant cross-reactivity or interference between Rat HMGB-1 and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: 18.75pg/mL

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat HMGB-1 concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HMGB-1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HMGB-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of HMGB-1. You can calculate the concentration of HMGB-1 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

41386729

ncbi acc num :

NP_788785.1

ncbi gb acc num :

NM_176612.1

uniprot acc num :

P10103

ncbi mol weight :

24,908 Da

ncbi pathways :

Activated TLR4 Signalling Pathway (1119352); Activation Of DNA Fragmentation Factor Pathway (1119617); Advanced Glycosylation Endproduct Receptor Signaling Pathway (1119372); Androgen Receptor Signaling Pathway (198553); Apoptosis Pathway (1119590); Apoptosis Induced DNA Fragmentation Pathway (1119616); Apoptotic Execution Phase Pathway (1119611); Base Excision Repair Pathway (84282); Base Excision Repair Pathway (451); Cytosolic Sensors Of Pathogen-associated DNA Pathway (1119381)

uniprot summary :

HMGB1: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Belongs to the HMGB family. Protein type: Nuclear receptor co-regulator; DNA repair, damage. Cellular Component: extracellular space; cell surface; transcriptional repressor complex; condensed chromosome; nucleus. Molecular Function: histone binding; double-stranded DNA binding; damaged DNA binding; chromatin binding; DNA bending activity; transcription factor activity; single-stranded DNA binding; chemoattractant activity. Biological Process: negative regulation of transcriptional preinitiation complex assembly; V(D)J recombination; DNA topological change; positive regulation of caspase activity; base-excision repair, DNA ligation; DNA recombination; chromatin remodeling; regulation of transcription from RNA polymerase II promoter; myeloid dendritic cell activation; inflammatory response to antigenic stimulus; positive chemotaxis; regulation of transcription, DNA-dependent; dendritic cell chemotaxis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of DNA binding

size1 :

48-Strip-Wells

price1 :

410 USD

size2 :

96-Strip-Wells

price2 :

490

more info or order :

MyBioSource product webpage