ELISA/assay

Porcine CKMB (Creatine Kinase MB Isoenzyme) ELISA Kit

MBS2500901

48-Strip-Wells

450 USD

ELISA Kit

Porcine CKMB (Creatine Kinase MB Isoenzyme) ELISA Kit

CKMB

creatine kinase M-type; Creatine kinase M-type; creatine kinase M-type; M-CK; creatine kinase M chain; creatine kinase, muscle; Creatine kinase M chain; M-CK

CKMB

Ckm; Ckm; Ckmm; Ckmm

KCRM_RAT

Porcine

381

This kit recognizes natural and recombinant Porcine CKMB. No significant cross-reactivity or interference between Porcine CKMB and analogues was observed.

Store at 4 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.234-15ng/mL. Sensitivity: The minimum detectable dose of Porcine CKMB is 0.141ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Porcine CKMB concentrations in serum, plasma and other biological fluids.

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CKMB. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CKMB and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CKMB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of CKMB. You can calculate the concentration of CKMB in the samples by comparing the OD of the samples to the standard curve.

6978661

NP_036662.1

NM_012530.2

P00564

43,045 Da

Arginine And Proline Metabolism Pathway (83349); Arginine And Proline Metabolism Pathway (323); Creatine Metabolism Pathway (1121093); Creatine Pathway (434676); Creatine Pathway (468234); Metabolic Pathways (132933); Metabolism Pathway (1120894); Metabolism Of Amino Acids And Derivatives Pathway (1121082); Urea Cycle And Metabolism Of Amino Groups Pathway (198443)

catalyzes regeneration of ATP; expression is induced by p53 [RGD, Feb 2006]

CKM: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family. Protein type: EC 2.7.3.2; Amino Acid Metabolism - arginine and proline; Kinase, other. Cellular Component: cytoplasm. Molecular Function: creatine kinase activity; ATP binding. Biological Process: phosphocreatine biosynthetic process; phosphorylation

48-Strip-Wells

450 USD

96-Strip-Wells

535