catalog number :
MBS2500783
products type :
ELISA Kit
products full name :
Human TYR (Tyrosinase) ELISA Kit
products short name :
TYR
other names :
TYR, partial; TYR; TYR
uniprot entry name :
F5CQG8_MEGNO
specificity :
This kit recognizes natural and recombinant Human TYR. No significant cross-reactivity or interference between Human TYR and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.625-40ng/mL. Sensitivity: The minimum detectable dose of Human TYR is 0.375ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
other info2 :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human TYR concentrations in serum, plasma and other biological fluids.
products description :
Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TYR. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TYR and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TYR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TYR. You can calculate the concentration of TYR in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
AEB71713.1
ncbi mol weight :
19,455 Da