Rat OPG (Osteoprotegerin) ELISA Kit | MyBioSource MBS2500776 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat OPG (Osteoprotegerin) ELISA Kit

catalog :

MBS2500776

quantity :

24-Strip-Wells

price :

190 USD

product information

catalog number :

MBS2500776

products type :

ELISA Kit

products full name :

Rat OPG (Osteoprotegerin) ELISA Kit

products short name :

[OPG]

other names :

[osteoprotegerin; Tumor necrosis factor receptor superfamily member 11B; tumor necrosis factor receptor superfamily member 11B; osteoclastogenesis inhibitory factor; osteoprotegerin; tumor necrosis factor receptor superfamily, member 11b; Osteoclastogenesis inhibitory factor; Osteoprotegerin]

products gene name :

[OPG]

other gene names :

[TNFRSF11B; TNFRSF11B; OPG; TR1; OCIF; OCIF; OPG]

uniprot entry name :

TR11B_HUMAN

reactivity :

Rat

sequence length :

401

specificity :

This kit recognizes Rat NT-proBNP in samples. No significant cross-reactivity or interference between Rat NT-proBNP and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: 18.75pg/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat NT-proBNP were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat NT-proBNP were tested on 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat NT-proBNP concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NT-proBNP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NT-proBNP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NT-proBNP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat NT-proBNP. You can calculate the concentration of Rat NT-proBNP in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

2072185

ncbi acc num :

AAB53709.1

uniprot acc num :

O00300

ncbi mol weight :

46,026 Da

ncbi pathways :

Apoptosis Modulation And Signaling Pathway (198822); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Monoamine Transport Pathway (198851); Osteoblast Signaling Pathway (198842); Osteoclast Signaling Pathway (198769); Osteoclast Differentiation Pathway (193147); Osteoclast Differentiation Pathway (193096); RANKL/RANK Signaling Pathway (672447)

ncbi summary :

The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. [provided by RefSeq, Jul 2008]

uniprot summary :

TNFRSF11B: Acts as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local RANKL/OPG ratio. May also play a role in preventing arterial calcification. May act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis. Defects in TNFRSF11B are the cause of juvenile Paget disease (JPD); also known as hyperostosis corticalis deformans juvenilis or hereditary hyperphosphatasia or chronic congenital idiopathic hyperphosphatasia. JPD is a rare autosomal recessive osteopathy that presents in infancy or early childhood. The disorder is characterized by rapidly remodeling woven bone, osteopenia, debilitating fractures, and deformities due to a markedly accelerated rate of bone remodeling throughout the skeleton. Approximately 40 cases of JPD have been reported worldwide. Unless it is treated with drugs that block osteoclast- mediated skeletal resorption, the disease can be fatal. Protein type: Secreted; Secreted, signal peptide; Inhibitor. Chromosomal Location of Human Ortholog: 8q24. Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region. Molecular Function: cytokine activity; receptor activity. Biological Process: response to drug; response to magnesium ion; extracellular matrix organization and biogenesis; negative regulation of odontogenesis of dentine-containing teeth; response to estrogen stimulus; apoptosis; response to arsenic; signal transduction; negative regulation of bone resorption; skeletal development; response to nutrient. Disease: Paget Disease, Juvenile

size1 :

24-Strip-Wells

price1 :

190 USD

size2 :

48-Strip-Wells

price2 :

310

size3 :

96-Strip-Wells

price3 :

355

size4 :

5x96-Strip-Wells

price4 :

1435

size5 :

10x96-Strip-Wells

price5 :

2805